Anti-CD204 antibody (ab217843)
Key features and details
- Rabbit polyclonal to CD204
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD204 antibody
See all CD204 primary antibodies -
Description
Rabbit polyclonal to CD204 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Human CD204 aa 200-300 conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P21757 -
Positive control
- Human glioma and esophageal cancer tissues. Human colon carcinoma lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab217843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/100 - 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Membrane glycoproteins implicated in the pathologic deposition of cholesterol in arterial walls during atherogenesis. Two types of receptor subunits exist. These receptors mediate the endocytosis of a diverse group of macromolecules, including modified low density lipoproteins (LDL). Isoform III does not internalize actetylated LDL. -
Tissue specificity
Isoform I, isoform II and isoform III are expressed in monocyte-derived macrophages. -
Sequence similarities
Contains 1 collagen-like domain.
Contains 1 SRCR domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 4481 Human
- Entrez Gene: 20288 Mouse
- Omim: 153622 Human
- SwissProt: P21757 Human
- SwissProt: P30204 Mouse
- Unigene: 147635 Human
- Unigene: 239291 Mouse
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Alternative names
- CD204 antibody
- CD204 antigen antibody
- CD24 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human glioma tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal cancer tissue, labeling CD204 using ab217843 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
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All lanes : Anti-CD204 antibody (ab217843) at 1/200 dilution
Lane 1 : Human colon carcinoma lysates
Lane 2 : rat brain lysates
Secondary
All lanes : goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution
Developed using the ECL technique.
Predicted band size: 41 kDa
Observed band size: 41 kDa12% gel
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab217843 has been referenced in 2 publications.
- Kai K et al. Oral Squamous Cell Carcinoma Contributes to Differentiation of Monocyte-Derived Tumor-Associated Macrophages via PAI-1 and IL-8 Production. Int J Mol Sci 22:N/A (2021). PubMed: 34502382
- Cheng C et al. The scavenger receptor SCARA1 (CD204) recognizes dead cells through spectrin. J Biol Chem 294:18881-18897 (2019). PubMed: 31653705