Key features and details
- Rat monoclonal [ER-MP23] to Macrophage specific lectin
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG2a
Product nameAnti-Macrophage specific lectin antibody [ER-MP23]
DescriptionRat monoclonal [ER-MP23] to Macrophage specific lectin
SpecificityDetects mature macrophages in connective tissues which are situated in the vicinity of epithelia e.g. salivary glands, capsule of lymph nodes, thymus and various other organs. The antibody blocks the mouse macrophage galactose-specific lectin (MMGL).
Tested applicationsSuitable for: Flow Cytmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation (Mouse macrophage cell lines).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 1% BSA
Concentration information loading...
Our Abpromise guarantee covers the use of ab15635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
RelevanceThe antigen is found on the surface of connective tissue macrophages and their precursor cells in bone marrow. Macrophage cell lines (J774-1.6, RAW309Cr.1, WR19M.1) corresponding to mature macrophages express high levels of the antigen .
Cellular localizationCell Membrane
- Macrophage asialoglycoprotein binding protein 1 antibody
- Macrophage galactose specific lectin 1 antibody
- MASGP BP antibody
Overlay histogram showing RAW 264.7 cells stained with ab15635 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab15635, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2a, kappa monoclonal [aRTK2758] (ab18450, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in RAW 264.7 cells fixed with 4% paraformaldehyde used under the same conditions.
Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
ab15635 has been referenced in 7 publications.
- Heine SJ et al. Intradermal delivery of Shigella IpaB and IpaD type III secretion proteins: kinetics of cell recruitment and antigen uptake, mucosal and systemic immunity, and protection across serotypes. J Immunol 192:1630-40 (2014). Mouse . PubMed: 24453241
- Martinez-Santibañez G et al. Imaging white adipose tissue with confocal microscopy. Methods Enzymol 537:17-30 (2014). PubMed: 24480339
- Cudejko C et al. p16INK4a-deficiency promotes IL-4-induced polarization and inhibits pro-inflammatory signaling in macrophages. Blood : (2011). IHC-FoFr ; Mouse . PubMed: 21636855
- Zhang W et al. Macrophage differentiation and polarization via phosphatidylinositol 3-kinase/Akt-ERK signaling pathway conferred by serum amyloid P component. J Immunol 187:1764-77 (2011). Flow Cyt ; Mouse . PubMed: 21753147
- Westcott DJ et al. MGL1 promotes adipose tissue inflammation and insulin resistance by regulating 7/4hi monocytes in obesity. J Exp Med 206:3143-56 (2009). IHC (PFA fixed) ; Mouse . PubMed: 19995956
- Xu W et al. The Nrf2 transcription factor protects from toxin-induced liver injury and fibrosis. Lab Invest 88:1068-78 (2008). PubMed: 18679376
- Lumeng CN et al. Phenotypic switching of adipose tissue macrophages with obesity is generated by spatiotemporal differences in macrophage subtypes. Diabetes 57:3239-46 (2008). PubMed: 18829989