Key features and details
- Rat monoclonal [ER-HR3] to Macrophages + Monocytes
- Suitable for: IHC-Fr, Flow Cyt, IHC-P
- Reacts with: Mouse
- Isotype: IgG2c
Product nameAnti-Macrophages + Monocytes antibody [ER-HR3]
DescriptionRat monoclonal [ER-HR3] to Macrophages + Monocytes
SpecificityThis antibody is reactive to the majority of mouse monocytes and a subset of mature resident macrophages, especially those located in hemopoietic organs. Whereas all other leukocytes are negative, up to 70% of circulating monocytes stain positive with this antibody. About 30% of bone marrow cells express the antigen. Other positive macrophages can be found in the splenic red pulp, in the mesentric lymphoid paracortex, interfollicular areas of Peyer's patches and bone marrow. Few positive cells are observed in the thymic cortex and the connective tissues of the gastro-intestinal tract, the dermis and the renal medulla. Epidermal Langerhans cells also express the antigen.
Tested applicationsSuitable for: IHC-Fr, Flow Cyt, IHC-Pmore details
Species reactivityReacts with: Mouse
Tissue/ cell preparation
This antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 0.1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesPurified antibody in 0.2µm filtered solution.
Primary antibody notesThis antibody can be used to identify and localize a very distinct macrophage subpopulation found in various mouse organs and its distribution pattern is unique compared to that from other macrophage-specific antibodies. The antibody is especially suitable for ontogenic studies because ER-HR3 positive macrophages are closely related to hemopoietic islands, especially at erythropoietic sites.
Our Abpromise guarantee covers the use of ab59697 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab18703 - Rat monoclonal IgG2c, is suitable for use as an isotype control with this antibody.
|IHC-P||Use at an assay dependent concentration.|
RelevanceMonocytes are produced by the bone marrow from haematopoietic stem cell precursors called monoblasts. Monocytes circulate in the bloodstream for about one to three days and then typically move into tissues throughout the body. They consist of between 3 to 8 percent of the leukocytes in the blood. In the tissues monocytes mature into different types of macrophages at different anatomical locations. Macrophages are a type of white blood cell derived from monocytes that engulf invading antigenic molecules, viruses, and microorganisms and then display fragments of the antigen to activate helper T cells; ultimately stimulating the production of antibodies against the antigen.
ab59697 has been referenced in 2 publications.
- Vidyasagar A et al. Tubular expression of heat-shock protein 27 inhibits fibrogenesis in obstructive nephropathy. Kidney Int 83:84-92 (2013). PubMed: 22971995
- Van Beneden K et al. Valproic acid attenuates proteinuria and kidney injury. J Am Soc Nephrol 22:1863-75 (2011). IHC-P ; Mouse . PubMed: 21868496