Key features and details
- Mouse monoclonal [9B10] to MAD1
- Suitable for: IP, ICC/IF, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Product nameAnti-MAD1 antibody [9B10]
See all MAD1 primary antibodies
DescriptionMouse monoclonal [9B10] to MAD1
Tested applicationsSuitable for: IP, ICC/IF, Flow Cytmore details
Unsuitable for: WB
Species reactivityReacts with: Human
Recombinant full length MAD1 protein (Human)
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.1% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab5783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 5 µg/ml.|
|ICC/IF||1/500 - 1/1000.|
|Flow Cyt||Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Sequence similaritiesBelongs to the MAD1 family.
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
ab5783 staining MAD1 in a human colon carcinoma cell by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/100 in 1% PBS BSA) for 1 hour at 37°C. A goat anti-mouse IgG polyclonal was used as the secondary antibody at a dilution of 1/100.
Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
ab5783 has been referenced in 4 publications.
- Petsalaki E et al. The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling. J Cell Biol 217:861-876 (2018). PubMed: 29362225
- Monda JK & Cheeseman IM Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes. Open Biol 8:N/A (2018). PubMed: 30257893
- Rajanala K et al. Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function. J Cell Sci 127:3505-20 (2014). PubMed: 24938596
- Hewitt L et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human . PubMed: 20624899