Anti-MAD1 antibody [9B10] (ab5783)
![Flow Cytometry - Anti-MAD1 antibody [9B10] (ab5783)](https://www.abcam.com/ps/products/5/ab5783/Images/ab5783-195393-anti-mad1-antibody-9b10-flow-cytometry.jpg "Flow Cytometry - Anti-MAD1 antibody [9B10] (ab5783)")
Key features and details
- Mouse monoclonal [9B10] to MAD1
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
-
Product name
Anti-MAD1 antibody [9B10]
See all MAD1 primary antibodies -
Description
Mouse monoclonal [9B10] to MAD1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Human
-
Immunogen
Recombinant full length MAD1 protein (Human)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
9B10 -
Isotype
IgG2b -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab5783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 0.1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
|
Application notes
Is unsuitable for WB.
Target
-
Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. -
Tissue specificity
Expressed weakly at G0/G1 and highly at late S and G2/M phase. -
Involvement in disease
Defects in MAD1L1 are involved in the development and/or progression of various types of cancer. -
Sequence similarities
Belongs to the MAD1 family. -
Post-translational
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore. - Information by UniProt
-
Database links
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 654838 Human
-
Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
Images
-
Flow Cytometry - Anti-MAD1 antibody [9B10] (ab5783)Overlay histogram showing HeLa cells stained with ab5783 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab5783, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H+L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
References (5)
ab5783 has been referenced in 5 publications.
- Petsalaki E et al. The ESCRT protein Chmp4c regulates mitotic spindle checkpoint signaling. J Cell Biol 217:861-876 (2018). PubMed: 29362225
- Monda JK & Cheeseman IM Dynamic regulation of dynein localization revealed by small molecule inhibitors of ubiquitination enzymes. Open Biol 8:N/A (2018). PubMed: 30257893
- Vanarsdall AL et al. CD147 Promotes Entry of Pentamer-Expressing Human Cytomegalovirus into Epithelial and Endothelial Cells. mBio 9:N/A (2018). PubMed: 29739904
- Rajanala K et al. Phosphorylation of nucleoporin Tpr governs its differential localization and is required for its mitotic function. J Cell Sci 127:3505-20 (2014). PubMed: 24938596
- Hewitt L et al. Sustained Mps1 activity is required in mitosis to recruit O-Mad2 to the Mad1-C-Mad2 core complex. J Cell Biol 190:25-34 (2010). ICC/IF ; Human . PubMed: 20624899
