Key features and details
- Rabbit polyclonal to MAD1
- Suitable for: WB, ICC/IF
- Reacts with: Saccharomyces cerevisiae
- Isotype: IgG
Product nameAnti-MAD1 antibody
See all MAD1 primary antibodies
DescriptionRabbit polyclonal to MAD1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Saccharomyces cerevisiae
Recombinant fusion protein (S. cerevisiae).
- S.cerevisiae whole cell lysate (see images).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab11691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100. Detects a band of approximately 83 kDa.|
|ICC/IF||Use at an assay dependent dilution.|
FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Sequence similaritiesBelongs to the MAD1 family.
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
All lanes : Anti-MAD1 antibody (ab11691) at 1/100 dilution
Lane 1 : Wild type S. cerevisiae whole cell lysate
Lane 2 : mad1 knockout S. cerevisiae whole cell lysate
Developed using the ECL technique.
Observed band size: 83 kDa why is the actual band size different from the predicted?
ab11691 at a 1/100 dilution staining MAD1 in S.cerevisiae by immunofluorescence.
ab11691 has not yet been referenced specifically in any publications.