- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-MAD1 antibody
See all MAD1 primary antibodies -
DescriptionRabbit polyclonal to MAD1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment containing a sequence corresponding to a region within amino acids 427-674 of Human MAD1. (Uniprot: Q9Y6D9)
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Positive control
- NT2D1 whole cell lysate, IMR32 whole cell lysate, U87-MG whole cell lysate, MCF-7 whole cell lysate and HBL435 xenograft tissue.
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General notesStore as concentrated solution
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab126148 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 83 kDa. | |
| IHC-P | 1/100 - 1/500. Preform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0) | |
| ICC/IF | 1/100 - 1/500. |
Target
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FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
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Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
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Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
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Sequence similaritiesBelongs to the MAD1 family.
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Post-translational
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
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Database links
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 654838 Human
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Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
Images
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Western blot - Anti-MAD1 antibody (ab126148)Anti-MAD1 antibody (ab126148) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg
Predicted band size: 83 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody (ab126148)ab126148, at 1/500 dilution, staining MAD1 in Formalin-fixed, Paraffin-embedded HBL435 xenograft by Immunohistochemistry. -
Immunocytochemistry/ Immunofluorescence - Anti-MAD1 antibody (ab126148)ab126148, at 1/500 dilution, staining MAD1 in Paraformaldehyde-fixed MCF-7 cells by Immunocytochemistry/Immunofluorescence.
Protocols
References
ab126148 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"