Anti-MAD1 antibody (ab126148)

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Immunocytochemistry/ Immunofluorescence - Anti-MAD1 antibody (ab126148)
  • Western blot - Anti-MAD1 antibody (ab126148)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody (ab126148)

Key features and details

  • Rabbit polyclonal to MAD1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-MAD1 antibody
    See all MAD1 primary antibodies

  • Description

    Rabbit polyclonal to MAD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment containing a sequence corresponding to a region within amino acids 427-674 of Human MAD1. (Uniprot: Q9Y6D9)

  • Positive control

    • NT2D1 whole cell lysate, IMR32 whole cell lysate, U87-MG whole cell lysate, MCF-7 whole cell lysate and HBL435 xenograft tissue.

  • General notes

    Store as concentrated solution

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
WB
Human
Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/500. Preform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF
1/100 - 1/500.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P
1/100 - 1/500. Preform antigen retrieval using heat mediated 10mM Citrate buffer(pH6.0) or Tris-EDTA buffer (pH8.0)
ICC/IF
1/100 - 1/500.

Target

  • Function

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

  • Tissue specificity

    Expressed weakly at G0/G1 and highly at late S and G2/M phase.

  • Involvement in disease

    Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

  • Sequence similarities

    Belongs to the MAD1 family.

  • Post-translational
    modifications

    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt

  • Database links

    • Alternative names

      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MAD1 antibody (ab126148)
      Immunocytochemistry/ Immunofluorescence - Anti-MAD1 antibody (ab126148)
      ab126148, at 1/500 dilution, staining MAD1 in Paraformaldehyde-fixed MCF-7 cells by Immunocytochemistry/Immunofluorescence.
    • Western blot - Anti-MAD1 antibody (ab126148)
      Western blot - Anti-MAD1 antibody (ab126148)
      Anti-MAD1 antibody (ab126148) at 1/1000 dilution + MCF-7 whole cell lysate at 30 µg

      Predicted band size: 83 kDa



      7.5% SDS PAGE
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody (ab126148)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody (ab126148)
      ab126148, at 1/500 dilution, staining MAD1 in Formalin-fixed, Paraffin-embedded HBL435 xenograft by Immunohistochemistry.

    References (0)

    Publishing research using ab126148? Please let us know so that we can cite the reference in this datasheet.

    ab126148 has not yet been referenced specifically in any publications.

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