- Datasheet
- Specific References
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Overview
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Product nameAnti-MAD1 antibody
See all MAD1 primary antibodies -
DescriptionRabbit polyclonal to MAD1
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Cow
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-367 of Human MAD1 (Q9Y6D9)
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Positive control
- 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji cell extracts, paraffin-embedded SW480 Xenograft
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.75% Glycine, 1.21% Tris, 10% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab155236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000 - 1/20000. Predicted molecular weight: 83 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
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Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
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Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
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Sequence similaritiesBelongs to the MAD1 family.
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Post-translational
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. -
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
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Database links
- Entrez Gene: 8379 Human
- Omim: 602686 Human
- SwissProt: Q9Y6D9 Human
- Unigene: 654838 Human
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Alternative names
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody (ab155236)Immunohistochemical analysis of paraffin-embedded SW480 Xenograft labeling MAD1 using ab155236 at 1/100 dilution. -
Western blot - Anti-MAD1 antibody (ab155236)Anti-MAD1 antibody (ab155236) at 1/10000 dilution + Molt-4 whole cell lysate at 30 µg
Developed using the ECL technique.
Predicted band size: 83 kDa
References
ab155236 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"