Key features and details
- Rabbit polyclonal to MAD1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MAD1 antibody
See all MAD1 primary antibodies
DescriptionRabbit polyclonal to MAD1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant full length protein corresponding to Human MAD1 aa 1-718.
MEDLGENTMVLSTLRSLNNFISQRVEGGSGLDISTSAPGSLQMQYQQSMQ LEERAEQIRSKSHLIQVEREKMQMELSHKRARVELERAASTSARNYEREV DRNQELLTRIRQLQEREAGAEEKMQEQLERNRQCQQNLDAASKRLREKED SLAQAGETINALKGRISELQWSVMDQEMRVKRLESEKQELQEQLDLQHKK CQEANQKIQELQASQEARADHEQQIKDLEQKLSLQEQDAAIVKNMKSELV RLPRLERELKQLREESAHLREMRETNGLLQEELEGLQRKLGRQEKMQETL VGLELENERLLAKLQSWERLDQTMGLSIRTPEDLSRFVVELQQRELALKD KNSAVTSSARGLEKARQQLQEELRQVSGQLLEERKKRETHEALARRLQKR VLLLTKERDGMRAILGSYDSELTPAEYSPQLTRRMREAEDMVQKVHSHSA EMEAQLSQALEELGGQKQRADMLEMELKMLKSQSSSAEQSFLFSREEADT LRLKVEELEGERSRLEEEKRMLEAQLERRALQGDYDQSRTKVLHMSLNPT SVARQRLREDHSQLQAECERLRGLLRAMERGGTVPADLEAAAASLPSSKE VAELKKQVESAELKNQRLKEVFQTKIQEFRKACYTLTGYQIDITTENQYR LTSLYAEHPGDCLIFKATSPSGSKMQLLETEFSHTVGELIEVHLRRQDSI PAFLSSLTLELFSRQTVA
Database link: Q9Y6D9
- HepG2 and HeLa cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab175245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/50 - 1/200.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
|WB||1/500 - 1/2000. Predicted molecular weight: 83 kDa.|
FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Sequence similaritiesBelongs to the MAD1 family.
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
Immunocytochemistry/Immunofluorescence analysis of HeLa cells using ab175245. Blue DAPI for nuclear staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue labelling MAD1 with ab175245 at 1/200. Magnification: 400x.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling MAD1 with ab175245 at 1/200. Magnification: 400x.
All lanes : Anti-MAD1 antibody (ab175245) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Predicted band size: 83 kDa
ab175245 has been referenced in 3 publications.
- Raab M et al. PLK1 has tumor-suppressive potential in APC-truncated colon cancer cells. Nat Commun 9:1106 (2018). PubMed: 29549256
- Cao L et al. AR-PDEF pathway promotes tumour proliferation and upregulates MYC-mediated gene transcription by promoting MAD1 degradation in ER-negative breast cancer. Mol Cancer 17:136 (2018). PubMed: 30217192
- Cunningham CE et al. Therapeutic relevance of the protein phosphatase 2A in cancer. Oncotarget 7:61544-61561 (2016). PubMed: 27557495