Key features and details
- Rabbit polyclonal to MAD1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAD1 antibody
See all MAD1 primary antibodies
DescriptionRabbit polyclonal to MAD1
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
A region between residues 1 and 50 of human MAD1 using the numbering given in Swiss-Prot entry Q9Y6D9.
- Human prostate adenocarcinoma tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to MAD1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab84490 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Epitope exposure is recommended.
Epitope exposure with citrate buffer will enhance staining.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionComponent of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
Tissue specificityExpressed weakly at G0/G1 and highly at late S and G2/M phase.
Involvement in diseaseDefects in MAD1L1 are involved in the development and/or progression of various types of cancer.
Sequence similaritiesBelongs to the MAD1 family.
modificationsPhosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
Cellular localizationNucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
- Information by UniProt
- hMAD1 antibody
- HsMAD1 antibody
- MAD1 antibody
ab84490 has not yet been referenced specifically in any publications.