RecombinantRabMAb

Recombinant Anti-MAD1 antibody [EPR14676-23] (ab184560)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] (ab184560)
  • Western blot - Anti-MAD1 antibody [EPR14676-23] (ab184560)
  • Anti-MAD1 antibody [EPR14676-23] (ab184560)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14676-23] to MAD1
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MAD1 antibody [EPR14676-23]
    See all MAD1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14676-23] to MAD1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HepG2, A431, HeLa and MCF7 lysates. Human melanoma tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR14676-23

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184560 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/10000 - 1/50000. Predicted molecular weight: 83 kDa.
IHC-P
1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.

  • Tissue specificity

    Expressed weakly at G0/G1 and highly at late S and G2/M phase.

  • Involvement in disease

    Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.

  • Sequence similarities

    Belongs to the MAD1 family.

  • Post-translational
    modifications

    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.

  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Information by UniProt

  • Database links

    • Alternative names

      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] (ab184560)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] (ab184560)

      Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1  with ab184560 at 1/50 dilution, counterstained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-MAD1 antibody [EPR14676-23] (ab184560)
      Western blot - Anti-MAD1 antibody [EPR14676-23] (ab184560)
      All lanes : Anti-MAD1 antibody [EPR14676-23] (ab184560) at 1/20000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : A431 lysate
      Lane 3 : HeLa lysate
      Lane 4 : MCF7 lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa

    • Anti-MAD1 antibody [EPR14676-23] (ab184560)
      Anti-MAD1 antibody [EPR14676-23] (ab184560)

    References (0)

    Publishing research using ab184560? Please let us know so that we can cite the reference in this datasheet.

    ab184560 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-MAD1 antibody [EPR14676-23]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Permeabilization
    No
    Specification
    HeLa
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
    Fixative
    Methanol
    Read More

    Abcam user community

    Verified customer

    Submitted May 23 2017

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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