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RecombinantRabMAb

Recombinant Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)

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  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
  • Western blot - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
  • Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14676-23] to MAD1 - BSA and Azide free
  • Suitable for: IHC-P, WB
  • Reacts with: Human

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Overview

  • Product name

    Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free
    See all MAD1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14676-23] to MAD1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human MAD1 aa 50-200. The exact sequence is proprietary.
    Database link: Q9Y6D9

  • General notes

    Ab250761 is the carrier-free version of ab184560. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab250761 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Affinity purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14676-23
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle

Associated products

  • Alternative Versions

    • Anti-MAD1 antibody [EPR14676-23] (ab184560)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • A431 whole cell lysate (ab7909)
  • Related Products

    • Recombinant Human Mad protein (ab82180)

Applications

Our Abpromise guarantee covers the use of ab250761 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 83 kDa.

Target

  • Function

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding.
  • Tissue specificity

    Expressed weakly at G0/G1 and highly at late S and G2/M phase.
  • Involvement in disease

    Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
  • Sequence similarities

    Belongs to the MAD1 family.
  • Post-translational
    modifications

    Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > microtubule organizing center > centrosome. Cytoplasm > cytoskeleton > spindle. From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Colocalizes with NEK2 at the kinetochore.
  • Target information above from: UniProt accession Q9Y6D9 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 8379 Human
    • Omim: 602686 Human
    • SwissProt: Q9Y6D9 Human
    • Unigene: 654838 Human
    • Alternative names

      • hMAD1 antibody
      • HsMAD1 antibody
      • MAD1 antibody
      • MAD1 mitotic arrest deficient like 1 (yeast) antibody
      • MAD1-like protein 1 antibody
      • MAD1L1 antibody
      • MD1L1_HUMAN antibody
      • Mitotic arrest deficient 1 antibody
      • Mitotic arrest deficient 1-like protein 1 antibody
      • Mitotic checkpoint MAD1 protein homolog antibody
      • Mitotic spindle assembly checkpoint protein MAD1 antibody
      • PIG9 antibody
      • Tax binding protein 181 antibody
      • Tax-binding protein 181 antibody
      • TP53I9 antibody
      • Tumor protein p53 inducible protein 9 antibody
      • TXBP181 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
      This data was developed using ab184560, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of formalin fixed and paraffin embedded Human melanoma tissue labeling MAD1 with ab184560 at 1/50 dilution, counterstained with Hematoxylin. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    • Western blot - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
      Western blot - Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
      All lanes : Anti-MAD1 antibody [EPR14676-23] (ab184560) at 1/20000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : A431 lysate
      Lane 3 : HeLa lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Developed using the ECL technique.

      Predicted band size: 83 kDa



      This data was developed using ab184560, the same antibody clone in a different buffer formulation.

       

    • Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)
      Anti-MAD1 antibody [EPR14676-23] - BSA and Azide free (ab250761)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab250761? Please let us know so that we can cite the reference in this datasheet.

    ab250761 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    There are currently no Customer reviews or Questions for ab250761.
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