• Product name
    Anti-Mad2L1 antibody [17D10]
    See all Mad2L1 primary antibodies
  • Description
    Mouse monoclonal [17D10] to Mad2L1
  • Host species
  • Specificity
    Western blot with a variety of human cell lines confirms that the antibody is specific for Mad2.
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant human Mad2.



Our Abpromise guarantee covers the use of ab10691 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 24.6 kDa).
IP Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
    • Sequence similarities
      Belongs to the MAD2 family.
      Contains 1 HORMA domain.
    • Domain
      The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
    • Post-translational
      Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
    • Cellular localization
      Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
    • Information by UniProt
    • Database links
    • Alternative names
      • HsMAD 2 antibody
      • HsMAD2 antibody
      • MAD 2 antibody
      • MAD 2A antibody
      • MAD2 like 1 antibody
      • MAD2 like protein 1 antibody
      • MAD2 mitotic arrest deficient like 1 antibody
      • MAD2-like protein 1 antibody
      • Mad2l1 antibody
      • MD2L1_HUMAN antibody
      • Mitotic arrest deficient 2-like protein 1 antibody
      • Mitotic arrest deficient yeast homolog like 1 antibody
      • Mitotic spindle assembly checkpoint protein MAD2A antibody
      see all


    • ab10691 staining Mad2L1 in colon cancer tissue, after antigen retreival, by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

    • Western blot using ab10691 at 1/500.

      Lane 1: Hela Nuclear
      Lane 2: Hela
      Lane 3: A431
      Lane 4: Jurkat
      Lane 5: 293

      Mad2L1 predicted size: 24.6kD.

      Secondary ab: Rabbit polyclonal to Mouse IgG H&L (HRP) ab6728 (1/5000)
      Exposure time: 1min
      20µg of lysate per lane.

      Western blot using ab10691 at 1/500. Lane 1: Hela Nuclear Lane 2: Hela Lane 3: A431 Lane 4: Jurkat Lane 5: 293 Mad2L1 predicted size: 24.6kD. Secondary ab: anti-mouse HRP (ab6728) (1/5000) Exposure time: 1min 20µg of lysate per lane.

    • Mad2L1 antibody (ab10691) at 1/1000 (in TBS for 90 minutes at 22°C) + whole tissue lysate of Human liver biopsy (chronic hepatitis C - (HepC) or non-viral (NVH)) patients at 50µg.

      Secondary Antibody: An HRP-conjugated Goat anti-mouse polyclonal (1/100000).

      Blocking Step: 5% Milk for 16 hours at 4°C

      See Abreview


    This product has been referenced in:
    • Alfieri C  et al. Mechanism for remodelling of the cell cycle checkpoint protein MAD2 by the ATPase TRIP13. Nature 559:274-278 (2018). Read more (PubMed: 29973720) »
    • Tambe M  et al. Novel Mad2-targeting miR-493-3p controls mitotic fidelity and cancer cells' sensitivity to paclitaxel. Oncotarget 7:12267-85 (2016). WB . Read more (PubMed: 26943585) »
    See all 5 Publications for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Human Tissue lysate - whole (Human liver biopsy)
    Loading amount
    50 µg
    Human liver biopsy
    Gel Running Conditions
    Reduced Denaturing (12%)
    Blocking step
    Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

    Dr. Saira Khalid

    Verified customer

    Submitted Sep 03 2010

    Abcam has not validated the combination of species/application used in this Abreview.
    Immunocytochemistry/ Immunofluorescence
    Human Cell (HeLa)

    Dr. Kirk Mcmanus

    Verified customer

    Submitted Dec 18 2006


    The epitope recognized by this antibody has not been mapped.

    Read More


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