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  1. Link

    mad2l1-antibody-epr9852-ab171084.pdf

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Cell Biology Cell Cycle Cell Division Spindle
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-Mad2L1 antibody [EPR9852] (ab171084)

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Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
  • Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
  • Anti-Mad2L1 antibody [EPR9852] (ab171084)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9852] to Mad2L1
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-Mad2L1 antibody [EPR9852]
    See all Mad2L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR9852] to Mad2L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human Mad2L1 aa 100-200. The exact sequence is proprietary.
    Database link: Q13257

  • Positive control

    • WB: HAP1, HeLa, 293T, Jurkat, Raji and A431 cell lysates;
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: Glycerol (glycerin, glycerine), BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR9852
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Tags & Cell Markers
    • Subcellular Markers
    • Nucleus
    • Other Nuclear Bodies
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Cancer
    • Cell cycle
    • Cell division

Associated products

  • Alternative Versions

    • Anti-Mad2L1 antibody [EPR9852] - BSA and Azide free (ab249639)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Recombinant Human Mad2L1 protein (ab113604)
    • Raji whole cell lysate (ab30124)
    • Jurkat whole cell lysate (ab30128)
    • Jurkat whole cell lysate (ab7899)
    • A-431 whole cell lysate (ab7909)
  • Recombinant Protein

    • Recombinant Human Mad2L1 protein (ab113604)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab171084 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 24 kDa.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 24 kDa.
Application notes
Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

Target

  • Function

    Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate.
  • Sequence similarities

    Belongs to the MAD2 family.
    Contains 1 HORMA domain.
  • Domain

    The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20.
  • Post-translational
    modifications

    Phosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20.
  • Cellular localization

    Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD.
  • Target information above from: UniProt accession Q13257 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4085 Human
    • Omim: 601467 Human
    • SwissProt: Q13257 Human
    • Unigene: 591697 Human
    • Alternative names

      • HsMAD 2 antibody
      • HsMAD2 antibody
      • MAD 2 antibody
      • MAD 2A antibody
      • MAD2 like 1 antibody
      • MAD2 like protein 1 antibody
      • MAD2 mitotic arrest deficient like 1 antibody
      • MAD2-like protein 1 antibody
      • Mad2l1 antibody
      • MD2L1_HUMAN antibody
      • Mitotic arrest deficient 2-like protein 1 antibody
      • Mitotic arrest deficient yeast homolog like 1 antibody
      • Mitotic spindle assembly checkpoint protein MAD2A antibody
      see all

    Images

    • Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
      Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
      All lanes : Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution

      Lane 1 : Wild-type HAP1 whole cell lysate
      Lane 2 : Mad2L1 knockout HAP1 whole cell lysate
      Lane 3 : HeLa whole cell lysate
      Lane 4 : A431 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 24 kDa
      Observed band size: 24 kDa



      Lanes 1 - 4: Merged signal (red and green). Green - ab171084 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab171084 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab171084 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
      Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)
      All lanes : Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : Raji cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 24 kDa

    • Anti-Mad2L1 antibody [EPR9852] (ab171084)
      Anti-Mad2L1 antibody [EPR9852] (ab171084)

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (0)

    Publishing research using ab171084? Please let us know so that we can cite the reference in this datasheet.

    ab171084 has not yet been referenced specifically in any publications.

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