Recombinant Anti-Mad2L1 antibody [EPR9852] (ab171084)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9852] to Mad2L1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-Mad2L1 antibody [EPR9852]
See all Mad2L1 primary antibodies -
Description
Rabbit monoclonal [EPR9852] to Mad2L1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human Mad2L1 aa 100-200. The exact sequence is proprietary.
Database link: Q13257 -
Positive control
- WB: HAP1, HeLa, 293T, Jurkat, Raji and A431 cell lysates;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: Glycerol, BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9852 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab171084 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 24 kDa. |
Target
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Function
Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Required for the execution of the mitotic checkpoint which monitors the process of kinetochore-spindle attachment and inhibits the activity of the anaphase promoting complex by sequestering CDC20 until all chromosomes are aligned at the metaphase plate. -
Sequence similarities
Belongs to the MAD2 family.
Contains 1 HORMA domain. -
Domain
The protein has two highly different native conformations, an inactive open conformation that cannot bind CDC20 and that predominates in cytosolic monomers, and an active closed conformation. The protein in the closed conformation preferentially dimerizes with another molecule in the open conformation, but can also form a dimer with a molecule in the closed conformation. Formation of a heterotetrameric core complex containing two molecules of MAD1L1 and of MAD2L1 in the closed conformation promotes binding of another molecule of MAD2L1 in the open conformation and the conversion of the open to the closed form, and thereby promotes interaction with CDC20. -
Post-translational
modificationsPhosphorylated on multiple serine residues. The level of phosphorylation varies during the cell cycle and is highest during mitosis. Phosphorylation abolishes interaction with MAD1L1 and reduces interaction with CDC20. -
Cellular localization
Nucleus. Chromosome > centromere > kinetochore. Cytoplasm. Recruited by MAD1L1 to unattached kinetochores (Probable). Recruited to the nuclear pore complex by TPR during interphase. Recruited to kinetochores in late prometaphase after BUB1, CENPF, BUB1B and CENPE. Kinetochore association requires the presence of NEK2. Kinetochore association is repressed by UBD. - Information by UniProt
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Database links
- Entrez Gene: 4085 Human
- Omim: 601467 Human
- SwissProt: Q13257 Human
- Unigene: 591697 Human
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Alternative names
- HsMAD 2 antibody
- HsMAD2 antibody
- MAD 2 antibody
see all
Images
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![Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)](https://www.abcam.com/ps/products/171/ab171084/Images/ab171084-367717-anti-mad2l1-epr9852-antibody-western-blot-knockout-hap1-mad2l1-hela-a431-human-lysates.jpg)
Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)All lanes : Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : Mad2L1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 24 kDa
Observed band size: 24 kDaLanes 1 - 4: Merged signal (red and green). Green - ab171084 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab171084 was shown to specifically react with in wild-type HAP1 cells as signal was lost in MAD2L1 knockout cells. Wild-type and MAD2L1 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab171084 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)](https://www.abcam.com/ps/products/171/ab171084/Images/ab171084-171526-anti-mad2l1-antibody-epr9852-western-blot.jpg)
Western blot - Anti-Mad2L1 antibody [EPR9852] (ab171084)All lanes : Anti-Mad2L1 antibody [EPR9852] (ab171084) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : Raji cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 24 kDa
Datasheets and documents
References (0)
ab171084 has not yet been referenced specifically in any publications.
