Anti-Mad2L2/REV7 antibody (ab115622)
Key features and details
- Rabbit polyclonal to Mad2L2/REV7
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Mad2L2/REV7 antibody
See all Mad2L2/REV7 primary antibodies -
Description
Rabbit polyclonal to Mad2L2/REV7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Horse, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
Synthetic peptide corresponding to Human Mad2L2/REV7 aa 1-100 conjugated to keyhole limpet haemocyanin.
Database link: NP_006332.2
(Peptide available asab11254) -
Positive control
- Human Brain, Cerebellum tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab115622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation. -
Tissue specificity
Ubiquitously expressed. -
Sequence similarities
Contains 1 HORMA domain. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10459 Human
- Entrez Gene: 71890 Mouse
- Entrez Gene: 313702 Rat
- Entrez Gene: 394380 Xenopus laevis
- Entrez Gene: 550258 Zebrafish
- Entrez Gene: 557968 Zebrafish
- Omim: 604094 Human
- SwissProt: Q9UI95 Human
see all -
Alternative names
- Homolog of REV7 S cerevisiae antibody
- hREV7 antibody
- MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
see all
Images
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ab115622 staining Mad2L2/REV7 in Human lung cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with the Hope Technique and blocked for 15 minutes at 25°C. Samples were incubated with primary antibody (1/300) for 1 hour at 25°C. An undiluted HRP-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.
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ab115622, at 10µg/ml, staining Mad2L2/REV7 in Formalin-fixed, Paraffin-embedded Human Brain Cerebellum tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab115622 has been referenced in 1 publication.
- Karakashev S et al. EZH2 Inhibition Sensitizes CARM1-High, Homologous Recombination Proficient Ovarian Cancers to PARP Inhibition. Cancer Cell 37:157-167.e6 (2020). PubMed: 32004442