Recombinant
RabMAb

Recombinant Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13657] to Mad2L2/REV7
  • Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Mad2L2/REV7 antibody [EPR13657]
    See all Mad2L2/REV7 primary antibodies

  • Description

    Rabbit monoclonal [EPR13657] to Mad2L2/REV7

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human Mad2L2/REV7 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UI95

  • Positive control

    • WB: K562, SW480, Hela, Molt-4 cell lysates. IHC-P: Human ovarian carcinoma tissue. ICC/IF: Hela cells. Flow Cyt: K562 cells IP: HeLa cell lysates

  • General notes

    This product was previously labelled as Mad2L2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13657

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
ICC/IF 1/100.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/50. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 24 kDa.

Target

  • Function

    Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.

  • Tissue specificity

    Ubiquitously expressed.

  • Sequence similarities

    Contains 1 HORMA domain.

  • Cellular localization

    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • Homolog of REV7 S cerevisiae antibody
    • hREV7 antibody
    • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
    • MAD2 homolog antibody
    • MAD2 like 2 antibody
    • MAD2 mitotic arrest deficient like 2 antibody
    • MAD2-like protein 2 antibody
    • MAD2B antibody
    • Mad2l2 antibody
    • MD2L2_HUMAN antibody
    • Mitotic Arrest Deficient 2 L2 antibody
    • Mitotic arrest deficient 2-like protein 2 antibody
    • Mitotic arrest deficient homolog like 2 antibody
    • Mitotic arrest deficient like 2 (yeast) antibody
    • Mitotic arrest deficient yeast homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD2B antibody
    • Polymerase (DNA directed) zeta 2 accessory subunit antibody
    • POLZ2 antibody
    • REV 7 antibody
    • REV7 antibody
    • REV7 homolog antibody
    • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
    see all

Images

  • Western blot - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Western blot - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    All lanes : Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunocytochemistry/ Immunofluorescence - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunoprecipitation - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunoprecipitation of HeLa cell lysate labeling Mad2L2/REV7 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

  • Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Flow Cytometry - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with ab180579 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/50.  

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

    Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG.  Counterstained with hematoxylin. Heat mediated antigen retrieval was performed with EDTA buffer pH 9 before commencing with IHC staining protocol.

References

Publishing research using ab180579? Please let us know so that we can cite the reference in this datasheet.

ab180579 has been referenced in 6 publications.

  • Li J  et al. Aurora B prevents aneuploidy via MAD2 during the first mitotic cleavage in oxidatively damaged embryos. Cell Prolif 52:e12657 (2019). PubMed: 31264311
  • Mirman Z  et al. 53BP1-RIF1-shieldin counteracts DSB resection through CST- and Pola-dependent fill-in. Nature 560:112-116 (2018). PubMed: 30022158
  • Chen W  et al. Effects of downregulated expression of microRNA-187 in gastric cancer. Exp Ther Med 16:1061-1070 (2018). PubMed: 30112051
  • Tonzi P  et al. Translesion polymerase kappa-dependent DNA synthesis underlies replication fork recovery. Elife 7:N/A (2018). PubMed: 30422114
  • Nascimento AV  et al. Overcoming cisplatin resistance in non-small cell lung cancer with Mad2 silencing siRNA delivered systemically using EGFR-targeted chitosan nanoparticles. Acta Biomater 47:71-80 (2017). PubMed: 27697601
  • Tang H  et al. MAD2B-mediated SnoN downregulation is implicated in fibroblast activation and tubulointerstitial fibrosis. Am J Physiol Renal Physiol 311:F207-16 (2016). PubMed: 27122545

Customer reviews and Q&As

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Western blot abreview for Anti-Mad2L2/REV7 antibody [EPR13657]

 Good
Abreviews
abreview image
Application
Western blot
Sample
Chicken Cell lysate - nuclear (DT40)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
100 µg
Specification
DT40
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jul 11 2016

Western blot abreview for Anti-Mad2L2 antibody [EPR13657]

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Lymphocytes)
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 29 2015

Western blot abreview for Anti-Mad2L2/REV7 antibody [EPR13657]

 Average
Abreviews
abreview image
Application
Western blot
Sample
Chicken Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Read More

Abcam user community

Verified customer

Submitted Jan 29 2015

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