Recombinant
RabMAb

Recombinant Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579)

Overview

  • Product name
    Anti-Mad2L2/REV7 antibody [EPR13657]
    See all Mad2L2/REV7 primary antibodies
  • Description
    Rabbit monoclonal [EPR13657] to Mad2L2/REV7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IP, ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Mad2L2/REV7 aa 150 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UI95

  • Positive control
    • K562, SW480, Hela, Molt-4 cell lysates, human ovarian carcinoma tissue, Hela cells,
  • General notes

     

     

     This product was previously labelled as Mad2L2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 0.05% BSA, 40% Glycerol
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR13657
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
ICC/IF 1/100.
Flow Cyt 1/70.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50.
WB 1/1000. Predicted molecular weight: 24 kDa.

Target

  • Function
    Adapter protein able to interact with different proteins and involved in different biological processes. Mediates the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1, thereby mediating the second polymerase switching in translesion DNA synthesis. Translesion DNA synthesis releases the replication blockade of replicative polymerases, stalled in presence of DNA lesions. May also regulate another aspect of cellular response to DNA damage through regulation of the JNK-mediated phosphorylation and activation of the transcriptional activator ELK1. Inhibits the FZR1- and probably CDC20-mediated activation of the anaphase promoting complex APC thereby regulating progression through the cell cycle. Regulates TCF7L2-mediated gene transcription and may play a role in epithelial-mesenchymal transdifferentiation.
  • Tissue specificity
    Ubiquitously expressed.
  • Sequence similarities
    Contains 1 HORMA domain.
  • Cellular localization
    Nucleus. Cytoplasm > cytoskeleton > spindle. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homolog of REV7 S cerevisiae antibody
    • hREV7 antibody
    • MAD2 (mitotic arrest deficient yeast, homolog) like 2 antibody
    • MAD2 homolog antibody
    • MAD2 like 2 antibody
    • MAD2 mitotic arrest deficient like 2 antibody
    • MAD2-like protein 2 antibody
    • MAD2B antibody
    • Mad2l2 antibody
    • MD2L2_HUMAN antibody
    • Mitotic Arrest Deficient 2 L2 antibody
    • Mitotic arrest deficient 2-like protein 2 antibody
    • Mitotic arrest deficient homolog like 2 antibody
    • Mitotic arrest deficient like 2 (yeast) antibody
    • Mitotic arrest deficient yeast homolog antibody
    • Mitotic spindle assembly checkpoint protein MAD2B antibody
    • Polymerase (DNA directed) zeta 2 accessory subunit antibody
    • POLZ2 antibody
    • REV 7 antibody
    • REV7 antibody
    • REV7 homolog antibody
    • Weakly similar to Mitotic MAD2 protein (S cerevisiae) antibody
    see all

Images

  • All lanes : Anti-Mad2L2/REV7 antibody [EPR13657] (ab180579) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : SW480 cell lysate
    Lane 3 : Hela cell lysate
    Lane 4 : Molt-4 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 24 kDa

  • Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling Mad2L2/REV7 with ab129199 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

    Control: PBS only.
    Nuclear counter stain: DAPI.

  • Immunoprecipitation of Hela cells labeling Mad2L2/REV7 using ab180579 at 1/40 dilution (lane 1) or negative control (lane 2) followed by anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500.  

  • Flow cytometric analysis of paraformadehyde fixed K562 cells labeling Mad2L2/REV7 with ab180579 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/50.  

  • Immunohistochemical staining of paraffin-embedded human ovarian carcinoma tissue labeling Mad2L2/REV7 using ab180579 at a 1/50 dilution and ImmunoHistoprobe HRP Polymer for Rabbit IgG.  Counterstained with hematoxylin.

References

This product has been referenced in:
  • Mirman Z  et al. 53BP1-RIF1-shieldin counteracts DSB resection through CST- and Pola-dependent fill-in. Nature 560:112-116 (2018). Read more (PubMed: 30022158) »
  • Nascimento AV  et al. Overcoming cisplatin resistance in non-small cell lung cancer with Mad2 silencing siRNA delivered systemically using EGFR-targeted chitosan nanoparticles. Acta Biomater 47:71-80 (2017). Read more (PubMed: 27697601) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application
Western blot
Sample
Chicken Cell lysate - nuclear (DT40)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
100 µg
Specification
DT40
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 11 2016

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12%)
Sample
Human Cell lysate - whole cell (Lymphocytes)
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 29 2015

Application
Western blot
Sample
Chicken Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
50 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jan 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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