The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Transcriptional repressor. Binds with MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. Antagonizes MYC transcriptional activity by competing for MAX and suppresses MYC dependent cell transformation.
Immunocytochemistry/ Immunofluorescence - Anti-MAD3 antibody (ab3995)This image is courtesy of an Abreview submitted by Dr Kirk McManus
ab3995 (1/200) staining MAD3 in HeLa cells (green). Cells were fixed in methanol, permeabilised in 0.5% Triton X-100/ PBS and counterstained with DAPI in order to highlight the nucleus (red). Please refer to abreview for further experimental details.