Key features and details
- Mouse monoclonal [314G8] to MAdCAM1
- Suitable for: Flow Cyt, IHC-P, ELISA, WB, Functional Studies, IHC-Fr
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MAdCAM1 antibody [314G8]
See all MAdCAM1 primary antibodies
DescriptionMouse monoclonal [314G8] to MAdCAM1
Tested applicationsSuitable for: Flow Cyt, IHC-P, ELISA, WB, Functional Studies, IHC-Frmore details
Species reactivityReacts with: Human
Recombinant soluble MAdCAM-1-fc fusion protein (Human).
Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesThis antibody was purified from tissue culture supernatant.
Primary antibody notesClone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).
Our Abpromise guarantee covers the use of ab34247 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||1/10 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
|IHC-P||Use at an assay dependent concentration.|
|ELISA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).|
|Functional Studies||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
- Information by UniProt
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
ab34247 has been referenced in 1 publication.
- Allavena R et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr ; Human . PubMed: 20035048