Overview

  • Product name
    Anti-MAdCAM1 antibody [314G8]
    See all MAdCAM1 primary antibodies
  • Description
    Mouse monoclonal [314G8] to MAdCAM1
  • Host species
    Mouse
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, ELISA, WB, Functional Studies, IHC-Frmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant soluble MAdCAM-1-fc fusion protein (Human).

  • General notes


    Clone 314G8 reacts with the ligand binding first Ig domain and reports suggest that a splice variant exists in the gut which is not recognised by clone 314G8. Clone 314G8 is reported to block the interaction of MAdCAM1 with alpha 4 beta 7 (see reference 1).

Properties

Applications

Our Abpromise guarantee covers the use of ab34247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 43 kDa).
Functional Studies Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function
    Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
  • Tissue specificity
    Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
  • Sequence similarities
    Contains 2 Ig-like (immunoglobulin-like) domains.
  • Post-translational
    modifications
    The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Addressin mucosal antibody
    • hMAdCAM 1 antibody
    • hMAdCAM-1 antibody
    • MACAM1 antibody
    • MADCA_HUMAN antibody
    • MAdCAM 1 antibody
    • MAdCAM-1 antibody
    • Madcam1 antibody
    • Mucosal addressin cell adhesion molecule 1 antibody
    • Mucosal vascular addressin cell adhesion molecule 1 antibody
    see all

References

This product has been referenced in:
  • Allavena R  et al. CNS elevation of vascular and not mucosal addressin cell adhesion molecules in patients with multiple sclerosis. Am J Pathol 176:556-62 (2010). IHC-Fr ; Human . Read more (PubMed: 20035048) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

The following paper describes production of the clone and characterization in three of the assays we list on the datasheet: Functional Assays, IHC-P, and WB

http://www.nature.com/icb/journal/v82/n4/full/icb200462a.html

Leung, E. et al. (2004) Bioassay detects soluble MAdCAM-1 in body fluids.
Immunol. Cell. Biol. 82: 400-409.

For flow cytometry testing, the sample was MAdCAM-transfected CHO cells.

Read More

Answer

Thank you for contacting us.



I am sorry to hear you are experiencing difficulties with ab34143 in IHC-Fr. This antibody is garanteed to work on Human samplesin IHC-Fr, Flow Cyt, ELISA, IHC-P andWB.

We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

I am attaching our questionnaire so that we can gather further information regarding the samples tested and the protocol used. Once we receive the completed questionnaire, we will look at the protocol and see if there are any suggestions we can make that may improve the results. This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

I look forward to receiving your reply.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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