Key features and details
- Rabbit polyclonal to MAdCAM1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAdCAM1 antibody
See all MAdCAM1 primary antibodies
DescriptionRabbit polyclonal to MAdCAM1
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human MAdCAM1 aa 19-317 (extracellular).
QSLQVKPLQVEPPEPVVAVALGASRQLTCRLACADRGASVQWRGLDTSLG AVQSDTGRSVLTVRNASLSAAGTRVCVGSCGGRTFQHTVQLLVYAFPDQL TVSPAALVPGDPEVACTAHKVTPVDPNALSFSLLVGGQELEGAQALGPEV QEEEEEPQGDEDVLFRVTERWRLPPLGTPVPPALYCQATMRLPGLELSHR QAIPVLHSPTSPEPPDTTSPESPDTTSPESPDTTSQEPPDTTSPEPPDKT SPEPAPQQGSTHTPRSPGSTRTRRPEISQAGPTQGEVIPTGSSKPAGDQ
Database link: Q13477
- WB: HepG2 whole cell lysate. ICC/IF: HepG2 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%.
Our Abpromise guarantee covers the use of ab237020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/2000. Predicted molecular weight: 40 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
- Information by UniProt
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
Anti-MAdCAM1 antibody (ab237020) at 1/200 dilution + HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for MAdCAM1 (green) using ab237020 at 1/100 dilution in ICC/IF, followed by an Alexa-Fluor®488 conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237020 has not yet been referenced specifically in any publications.