Anti-MAdCAM1 antibody [AP-MAB0842] (ab80680)
Key features and details
- Rat monoclonal [AP-MAB0842] to MAdCAM1
- Suitable for: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assay
- Reacts with: Mouse
- Isotype: IgG2
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MAdCAM1 antibody [AP-MAB0842]
See all MAdCAM1 primary antibodies -
Description
Rat monoclonal [AP-MAB0842] to MAdCAM1 -
Host species
Rat -
Tested applications
Suitable for: IHC-Fr, IP, IHC-P, Flow Cyt, Inhibition Assaymore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Mouse Endothelial cells
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General notes
Endotoxin Level: Extremely low level of LPS (< 0.002EU/µg IgG).ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
The IgG fraction of culture supernatant was purified by Protein A affinity chromatography. -
Primary antibody notes
ab80680 was produced by in vitro culture, from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
ab80680 is useful for reducing T cell mediated inflammation in gastrointestinal diseases. -
Clonality
Monoclonal -
Clone number
AP-MAB0842 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab80680 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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Inhibition Assay |
Use at an assay dependent concentration.
ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
Inhibition Assay
Use at an assay dependent concentration. ab80680 is useful for blocking the interaction of MAdCAM1 with its counter receptor. |
Target
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Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
Tissue specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 17123 Mouse
- SwissProt: Q61826 Mouse
- Unigene: 391556 Mouse
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Alternative names
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab80680 has been referenced in 1 publication.
- Gao RY et al. Bile acids modulate colonic MAdCAM-1 expression in a murine model of combined cholestasis and colitis. Mucosal Immunol 14:479-490 (2021). PubMed: 33004979