Product nameAnti-MAdCAM1 antibody [EPR6946]
See all MAdCAM1 primary antibodies
DescriptionRabbit monoclonal [EPR6946] to MAdCAM1
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
Species reactivityReacts with: Human
Synthetic peptide within Human MAdCAM1 aa 1-100 (extracellular). The exact sequence is proprietary.
- HeLa, HUVEC, and ECV-304 cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 2.84 x 10 -11 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab134088 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).|
FunctionCell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
Tissue specificityHighly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
Sequence similaritiesContains 2 Ig-like (immunoglobulin-like) domains.
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
- Information by UniProt
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
All lanes : Anti-MAdCAM1 antibody [EPR6946] (ab134088) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution
Predicted band size: 40 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
ab134088 has not yet been referenced specifically in any publications.