Recombinant Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
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Overview
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Product name
Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free
See all MAdCAM1 primary antibodies -
Description
Rabbit monoclonal [EPR6946] to MAdCAM1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MAdCAM1 aa 1-100 (extracellular). The exact sequence is proprietary.
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General notes
ab248677 is the carrier-free version of ab134088 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248677 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Dissociation constant (KD)
KD = 2.84 x 10 -11 M Learn more about KD -
Storage buffer
Constituent: PBS -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR6946 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab248677 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa). |
Target
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Function
Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding. -
Tissue specificity
Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes. -
Sequence similarities
Contains 2 Ig-like (immunoglobulin-like) domains. -
Post-translational
modificationsThe Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 8174 Human
- Omim: 102670 Human
- SwissProt: Q13477 Human
- Unigene: 102598 Human
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Alternative names
- Addressin mucosal antibody
- hMAdCAM 1 antibody
- hMAdCAM-1 antibody
see all
Datasheets and documents
References
ab248677 has not yet been referenced specifically in any publications.
