RecombinantRabMAb

Recombinant Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)

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Western blot - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
  • OI-RD Scanning - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
  • Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6946] to MAdCAM1 - BSA and Azide free
  • Suitable for: WB
  • Reacts with: Human

Overview

  • Product name

    Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free
    See all MAdCAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR6946] to MAdCAM1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MAdCAM1 aa 1-100 (extracellular). The exact sequence is proprietary.

  • General notes

    ab248677 is the carrier-free version of ab134088 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248677 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab248677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.

    • Target

    • Function

      Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.

    • Tissue specificity

      Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.

    • Sequence similarities

      Contains 2 Ig-like (immunoglobulin-like) domains.

    • Post-translational
      modifications

      The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • Addressin mucosal antibody
        • hMAdCAM 1 antibody
        • hMAdCAM-1 antibody
        • MACAM1 antibody
        • MADCA_HUMAN antibody
        • MAdCAM 1 antibody
        • MAdCAM-1 antibody
        • Madcam1 antibody
        • Mucosal addressin cell adhesion molecule 1 antibody
        • Mucosal vascular addressin cell adhesion molecule 1 antibody
        see all

      Images

      • Western blot - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
        Western blot - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
        All lanes : Anti-MAdCAM1 antibody [EPR6946] (ab134088) at 1/1000 dilution

        Lane 1 : HeLa cell lysate
        Lane 2 : HUVEC cell lysate
        Lane 3 : ECV-304 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled Goat anti-rabbit at 1/2000 dilution

        Predicted band size: 40 kDa
        Observed band size: 60 kDa
        why is the actual band size different from the predicted?



        This data was developed using ab134088, the same antibody clone in a different buffer formulation.

      • OI-RD Scanning - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
        OI-RD Scanning - Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
        This data was developed using ab134088, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
        Learn more about KD

        Click here to learn more about KD
      • Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)
        Anti-MAdCAM1 antibody [EPR6946] - BSA and Azide free (ab248677)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (0)

      Publishing research using ab248677? Please let us know so that we can cite the reference in this datasheet.

      ab248677 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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