Overview

  • Product name
    Anti-MAdCAM1 antibody [MECA-367] (FITC)
    See all MAdCAM1 primary antibodies
  • Description
    Rat monoclonal [MECA-367] to MAdCAM1 (FITC)
  • Host species
    Rat
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Tested applications
    Suitable for: Blocking, Flow Cyt, IHC-Fr, IP, WB, Othermore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation: Endothelial cells isolated from BALB/c mouse mesenteric and peripheral lymph nodes.

  • Epitope
    This antibody reacts with an epitope located in the first domain of MAdCAM1.

Properties

Applications

Our Abpromise guarantee covers the use of ab24941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Blocking
Flow Cyt
IHC-Fr
IP
WB
AP
Other
  • Application notes
    AP: Use at an assay dependent dilution.
    BL: Use at an assay dependent dilution.
    Note: This antibody is useful for in vitro cellular binding blocking, and for blocking lymphocyte homing to Peyers patch HEV in vivo.
    Flow Cyt: Use at an assay dependent dilution.
    IHC-Fr: Use at a concentration of 1 µg/ml.
    IP: Use at an assay dependent dilution.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 44 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Cell adhesion leukocyte receptor expressed by mucosal venules, helps to direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria. It can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes. Isoform 2, lacking the mucin-like domain, may be specialized in supporting integrin alpha-4/beta-7-dependent adhesion strengthening, independent of L-selectin binding.
    • Tissue specificity
      Highly expressed on high endothelial venules (HEV) and lamina propia venules found in the small intestine, and to a lesser extent in the colon and spleen. Very low levels of expression found in pancreas and brain. Not expressed in the thymus, prostate, ovaries, testis, heart, placenta, lung, liver, skeletal muscle, kidney or peripheral blood leukocytes.
    • Sequence similarities
      Contains 2 Ig-like (immunoglobulin-like) domains.
    • Post-translational
      modifications
      The Ser/Thr-rich mucin-like domain may provide possible sites for O-glycosylation.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Addressin mucosal antibody
      • hMAdCAM 1 antibody
      • hMAdCAM-1 antibody
      • MACAM1 antibody
      • MADCA_HUMAN antibody
      • MAdCAM 1 antibody
      • MAdCAM-1 antibody
      • Madcam1 antibody
      • Mucosal addressin cell adhesion molecule 1 antibody
      • Mucosal vascular addressin cell adhesion molecule 1 antibody
      see all

    References

    This product has been referenced in:
    • Hwang IY  et al. The loss of Gnai2 and Gnai3 in B cells eliminates B lymphocyte compartments and leads to a hyper-IgM like syndrome. PLoS One 8:e72596 (2013). IHC-Fr ; Mouse . Read more (PubMed: 23977324) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you very much for your interest in Abcam and our products..

    To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated as soon as we receive any new information.

    ab25644
    ab24941
    ab86900
    ab41467,
    ab60077,
    ab25175,
    ab24985,
    ab59437,
    ab116223

    Therefore, I can offer a discount off a future purchase if you buythese untested antibodies now, test in IHC-P and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback.One discount code would be provided for each individualantibody thatwould be to the value of 1 free primary antibody.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed.If you could let me know whichparticular antibodies youwant to test. I will then send a discount code for each of these antibody. This code must be issued before purchasing the antibodiesso please wait for my reply before ordering.

    2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

    3. Test it in IHC-P.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the any of the antibodiesturns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

    The following antibodies you listed have already been tested in IHC-P, and so we are not able to provide a testing discount for these on this occasion. As they have been tested in IHC-P, we can guarantee them for fluorescent studies on paraffin embedded tissue as well.

    ab112470
    ab61219
    ab6564

    We do not charge individual shipping for each item. We would charge one shipping fee for eachorder. Extra shipping charges are applied if there is a bulky item such as a kit, or a dry ice item on the order.

    With regards to discounts for new laboratories, I have spoken to our marketing department and believe thatone ofyour colleagues, Dr. ##### has already been in touch with us. A 25% discount has been arranged for orders from his laboratory for the next 1 month. Could you confirm that you are working in the same laboratories? I hope this discount will prove helpful in setting up the new laboratory. As we have already arranged these 2 types of discount, I am sorry we are not able to offer a further bulk discount or shipping discount on this occasion.

    I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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