Anti-MADH7/SMAD7 antibody (ab216428)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: ICC/IF, WB, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
-
Product name
Anti-MADH7/SMAD7 antibody
See all MADH7/SMAD7 primary antibodies -
Description
Rabbit polyclonal to MADH7/SMAD7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human MADH7/SMAD7 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: O15105 -
Positive control
- IHC-P: Rat kidney and lung tissue, mouse kidney tissue. WB: Mouse lung, lymph, spleen, stomach, large intestine and placenta lysates. Human HepG2 cell line. Rat cerebrum. ICC: U-2OS human cells. Flow Cyt: SH-SY5Y human cell line.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216428 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/100.
|
|
WB | (1) |
1/100 - 1/1000. Predicted molecular weight: 46 kDa.
|
IHC-P |
1/100 - 1/500.
|
|
Flow Cyt |
1/200.
|
Notes |
---|
ICC/IF
1/100. |
WB
1/100 - 1/1000. Predicted molecular weight: 46 kDa. |
IHC-P
1/100 - 1/500. |
Flow Cyt
1/200. |
Target
-
Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. -
Tissue specificity
Ubiquitous with higher expression in the lung and vascular endothelium. -
Involvement in disease
Colorectal cancer 3 -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1. -
Cellular localization
Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). - Information by UniProt
-
Database links
- Entrez Gene: 4092 Human
- Entrez Gene: 17131 Mouse
- Entrez Gene: 81516 Rat
- Omim: 602932 Human
- SwissProt: O15105 Human
- SwissProt: O35253 Mouse
- SwissProt: O88406 Rat
- Unigene: 465087 Human
see all -
Alternative names
- CRCS3 antibody
- FLJ16482 antibody
- hSMAD 7 antibody
see all
Images
-
All lanes : Anti-MADH7/SMAD7 antibody (ab216428) at 1/200 dilution
Lane 1 : SHSY5Y human cells
Lane 2 : HepG2 cells
Lane 3 : Mouse cerebrum
Lane 4 : Rat cerebrum
Lane 5 : Mouse stomach
Lane 6 : Mouse lung
Lane 7 : Mouse kidney
Secondary
All lanes : Conjugated Secondary at 1/200 dilution
Predicted band size: 46 kDa -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling MADH7/SMAD7 with ab216428 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
-
Flow cytometry analysis of SH-SY5Y cells labelled for MADH7/ SMAD7 using ab216428 at 1/200 dilution. Acquisition of 20,000 events were performed. Cells stained with primary antibody (green), isotype control (orange).
-
All lanes : Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution
Lane 1 : Mouse lung tissue lysate
Lane 2 : Mouse placenta tissue lysate
Lane 3 : Mouse large intestine tissue lysate
Secondary
All lanes : Conjugated secondary antibody at 1/20000 dilution
Predicted band size: 46 kDaPrimary incubation: 4°C overnight followed by conjugated secondary antibody incubation for 60 min at 37°C.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling MADH7/SMAD7 with ab216428 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
-
Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling MADH7/SMAD7 with ab216428 at 1/600 dilution followed by conjugation to the secondary antibody for 20 minutes and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min. Endogenous peroxidase was blocked by 3% hydrogen peroxide for 20 minutes and blocking buffer (normal goat serum) added at 37°C for 30min.
-
Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + mouse lymph node at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted? -
Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse spleen lysate at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 46 kDa -
Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse stomach lysate at 30 µg
Secondary
IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (37)
ab216428 has been referenced in 37 publications.
- Huang Y et al. RNF12 is regulated by AKT phosphorylation and promotes TGF-β driven breast cancer metastasis. Cell Death Dis 13:44 (2022). PubMed: 35013159
- Liu Y et al. Bone marrow mesenchymal stem cell-derived exosomes improve renal fibrosis via regulating Smurf 2/Smad 7. Front Biosci (Landmark Ed) 27:17 (2022). PubMed: 35090322
- Jin D et al. Impact of klotho on the expression of SRGAP2a in podocytes in diabetic nephropathy. BMC Nephrol 23:151 (2022). PubMed: 35436879
- He L et al. Co-overexpression of VEGF and Smad7 improved the therapeutic effects of adipose-derived stem cells on neurogenic erectile dysfunction in the rat model. Andrologia 54:e14538 (2022). PubMed: 35912795
- Ling J et al. A Regulatory Network Analysis of the Importance of USP15 in Breast Cancer Metastasis and Prognosis. J Oncol 2022:1427726 (2022). PubMed: 36213818