Overview

  • Product name

  • Description

    Rabbit polyclonal to MADH7/SMAD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide within Human MADH7/SMAD7 aa 1-50 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: O15105

  • Positive control

    • IHC-P: Rat kidney and lung tissue. WB: Mouse lung, lymph, spleen, stomach and placenta lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab216428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 46 kDa.
IHC-P 1/100 - 1/500.

Target

  • Function

    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • Tissue specificity

    Ubiquitous with higher expression in the lung and vascular endothelium.
  • Involvement in disease

    Colorectal cancer 3
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
    Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
    Acetylation prevents ubiquitination and degradation mediated by SMURF1.
  • Cellular localization

    Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).
  • Information by UniProt
  • Database links

  • Alternative names

    • CRCS3 antibody
    • FLJ16482 antibody
    • hSMAD 7 antibody
    • hSMAD7 antibody
    • MAD (mothers against decapentaplegic Drosophila) homolog 7 antibody
    • MAD antibody
    • Mad homolog 7 antibody
    • MAD homolog 8 antibody
    • MAD mothers against decapentaplegic homolog 7 antibody
    • MADH 7 antibody
    • MADH 8 antibody
    • MADH6 antibody
    • MADH7 antibody
    • MADH8 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody
    • Mothers against decapentaplegic homolog 7 antibody
    • Mothers against decapentaplegic homolog 8 antibody
    • Mothers against DPP homolog 7 antibody
    • Mothers against DPP homolog 8 antibody
    • SMA- AND MAD-RELATED PROTEIN 7 antibody
    • SMAD 7 antibody
    • SMAD antibody
    • SMAD family member 7 antibody
    • SMAD, mothers against DPP homolog 7 (Drosophila) antibody
    • SMAD, mothers against DPP homolog 7 antibody
    • SMAD6 antibody
    • Smad7 antibody
    • SMAD7_HUMAN antibody
    see all

Images

  • Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + mouse lymph node at 30 µg

    Secondary
    IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa
    why is the actual band size different from the predicted?

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling MADH7/SMAD7 with ab216428 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

     

  • Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse spleen lysate at 30 µg

    Secondary
    IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 46 kDa

  • Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution + Mouse stomach lysate at 30 µg

    Secondary
    IRDye800CW Goat Anti-Rabbit IgG at 1/2000 dilution

    Predicted band size: 46 kDa
    Observed band size: 50 kDa why is the actual band size different from the predicted?

  • Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution (overnight in 4°C) + mouse lung lysate

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated (90min in 37°C) at 1/5000 dilution

    Predicted band size: 46 kDa

  • Anti-MADH7/SMAD7 antibody (ab216428) at 1/300 dilution (overnight in 4°C) + mouse placenta lysate

    Secondary
    Goat anti-rabbit IgG (H+L), HRP conjugated (90min in 37°C) at 1/5000 dilution

    Predicted band size: 46 kDa

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat lung tissue labeling MADH7/SMAD7 with ab216428 at 1/600 dilution followed by conjugation to the secondary antibody for 20 minutes and DAB staining. Prior to addition of the primary antibody, antigen retrieval was carried out by boiling in sodium citrate buffer (pH6.0) for 15min.  Endogenous peroxidase was blocked by 3% hydrogen peroxide for 20 minutes and blocking buffer (normal goat serum) added at 37°C for 30min.

References

This product has been referenced in:

  • Yuan CL  et al. Bone morphogenetic protein and activin membrane-bound inhibitor overexpression inhibits gastric tumor cell invasion via the transforming growth factor-ß/epithelial-mesenchymal transition signaling pathway. Exp Ther Med 15:5422-5430 (2018). Read more (PubMed: 29805551) »
  • Liang ZG  et al. MicroRNA-20b-5p promotes ventricular remodeling by targeting the TGF-ß/Smad signaling pathway in a rat model of ischemia-reperfusion injury. Int J Mol Med 42:975-987 (2018). Read more (PubMed: 29786750) »
See all 3 Publications for this product

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