Overview

  • Product name

  • Description

    Rabbit polyclonal to MADH7/SMAD7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Pig
  • Immunogen

    Recombinant fragment within Human MADH7/SMAD7 (internal sequence). The exact sequence is proprietary.
    Database link: O15105

  • Positive control

    • WB: DDDDK-tagged MADH7/SMAD7 transfected HEK-293T whole cell extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab226872 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.

Target

  • Function

    Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
  • Tissue specificity

    Ubiquitous with higher expression in the lung and vascular endothelium.
  • Involvement in disease

    Colorectal cancer 3
  • Sequence similarities

    Belongs to the dwarfin/SMAD family.
    Contains 1 MH1 (MAD homology 1) domain.
    Contains 1 MH2 (MAD homology 2) domain.
  • Post-translational
    modifications

    Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
    Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
    Acetylation prevents ubiquitination and degradation mediated by SMURF1.
  • Cellular localization

    Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).
  • Information by UniProt
  • Database links

  • Alternative names

    • CRCS3 antibody
    • FLJ16482 antibody
    • hSMAD 7 antibody
    • hSMAD7 antibody
    • MAD (mothers against decapentaplegic Drosophila) homolog 7 antibody
    • MAD antibody
    • Mad homolog 7 antibody
    • MAD homolog 8 antibody
    • MAD mothers against decapentaplegic homolog 7 antibody
    • MADH 7 antibody
    • MADH 8 antibody
    • MADH6 antibody
    • MADH7 antibody
    • MADH8 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 6 antibody
    • Mothers Against Decapentaplegic Drosophila Homolog of 7 antibody
    • Mothers against decapentaplegic homolog 7 antibody
    • Mothers against decapentaplegic homolog 8 antibody
    • Mothers against DPP homolog 7 antibody
    • Mothers against DPP homolog 8 antibody
    • SMA- AND MAD-RELATED PROTEIN 7 antibody
    • SMAD 7 antibody
    • SMAD antibody
    • SMAD family member 7 antibody
    • SMAD, mothers against DPP homolog 7 (Drosophila) antibody
    • SMAD, mothers against DPP homolog 7 antibody
    • SMAD6 antibody
    • Smad7 antibody
    • SMAD7_HUMAN antibody
    see all

Images

  • All lanes : Anti-MADH7/SMAD7 antibody (ab226872) at 1/3000 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : DDDDK-tagged MADH7/SMAD7 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 46 kDa



    10% SDS-PAGE gel.

References

ab226872 has not yet been referenced specifically in any publications.

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