Anti-MADH7/SMAD7 antibody (ab244424)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MADH7/SMAD7 antibody
See all MADH7/SMAD7 primary antibodies -
Description
Rabbit polyclonal to MADH7/SMAD7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment corresponding to Human MADH7/SMAD7 aa 173-295.
Sequence:EVKRLCCCESYGKINPELVCCNPHHLSRLCELESPPPPYSRYPMDFLKPT ADCPDAVPSSAETGGTNYLAPGGLSDSQLLLEPGDRSHWCVVAYWEEKTR VGRLYCVQEPSLDIFYDLPQGNG
Database link: O15105 -
Positive control
- IHC-P: Human lung, placenta and colon tissues. ICC/IF: U-251 MG cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab244424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
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| IHC-P | 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. -
Tissue specificity
Ubiquitous with higher expression in the lung and vascular endothelium. -
Involvement in disease
Colorectal cancer 3 -
Sequence similarities
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
Post-translational
modificationsPhosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1. -
Cellular localization
Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). - Information by UniProt
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Database links
- Entrez Gene: 4092 Human
- Entrez Gene: 17131 Mouse
- Entrez Gene: 81516 Rat
- Omim: 602932 Human
- SwissProt: O15105 Human
- SwissProt: O35253 Mouse
- SwissProt: O88406 Rat
- Unigene: 465087 Human
see all -
Alternative names
- CRCS3 antibody
- FLJ16482 antibody
- hSMAD 7 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MADH7/SMAD7 antibody (ab244424)Paraffin-embedded human lung tissue stained for MADH7/SMAD7 using ab244424 at 1/50 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MADH7/SMAD7 antibody (ab244424)Paraffin-embedded human liver tissue stained for MADH7/SMAD7 using ab244424 at 1/50 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MADH7/SMAD7 antibody (ab244424)Paraffin-embedded human colon tissue stained for MADH7/SMAD7 using ab244424 at 1/50 dilution in immunohistochemical analysis. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MADH7/SMAD7 antibody (ab244424)Paraffin-embedded human placenta tissue stained for MADH7/SMAD7 using ab244424 at 1/50 dilution in immunohistochemical analysis. -
Immunocytochemistry/ Immunofluorescence - Anti-MADH7/SMAD7 antibody (ab244424)PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for MADH7/SMAD7 (green) using ab244424 at 4 µg/ml in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab244424 has not yet been referenced specifically in any publications.
