Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MADH7/SMAD7 antibody
See all MADH7/SMAD7 primary antibodies
DescriptionRabbit polyclonal to MADH7/SMAD7
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MADH7/SMAD7 (C terminal). NM_005904
Database link: O15105
- WB: HeLa cell lysate.
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FormLyophilized:Reconstitute with 200ul distilled H2O. For extended storage, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50%.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab90086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/200 - 1/1000. Predicted molecular weight: 46 kDa.|
FunctionAntagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Tissue specificityUbiquitous with higher expression in the lung and vascular endothelium.
Involvement in diseaseColorectal cancer 3
Sequence similaritiesBelongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain.
modificationsPhosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1.
Cellular localizationNucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236).
- Information by UniProt
- CRCS3 antibody
- FLJ16482 antibody
- hSMAD 7 antibody
ab90086 has been referenced in 13 publications.
- Jiao H et al. LncRNA PRINS is involved in the development of nephropathy in patients with diabetes via interaction with Smad7. Exp Ther Med 17:3203-3208 (2019). PubMed: 30936994
- Huang C et al. The role and mechanism of microRNA-18a-5p in oral squamous cell carcinoma. Mol Med Rep 20:1637-1644 (2019). PubMed: 31257489
- Xu T et al. Kaempferol attenuates liver fibrosis by inhibiting activin receptor-like kinase 5. J Cell Mol Med 23:6403-6410 (2019). PubMed: 31273920
- Yu Y et al. MiR-490-5p inhibits the stemness of hepatocellular carcinoma cells by targeting ECT2. J Cell Biochem 120:967-976 (2019). PubMed: 30206962
- Zhuang L et al. Co-culturing with hypoxia pre-conditioned mesenchymal stem cells as a new strategy for the prevention of irradiation-induced fibroblast-to-myofibroblast transition. Oncol Rep 42:1781-1792 (2019). PubMed: 31485596