Overview

  • Product name

    Anti-MADM antibody
  • Description

    Rabbit polyclonal to MADM
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cow, Pig, Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 20-353 of Human MADM (UniProt ID: Q9UHY1).

  • Positive control

    • Jurkat and Raji whole cell lysates; Mouse brain tissue lysate; A431 cells; Human breast carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154048 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May play a role in subcellular trafficking between the endoplasmic reticulum and Golgi apparatus through interactions with the Rho-type GTPases. Binding to the NS3 protein of dengue virus type 2 appears to subvert this activity into the alteration of the intracellular membrane structure associated with flaviviral replication.
  • Tissue specificity

    Ubiquitously expressed in all tissues examined with high levels in the testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Cellular localization

    Cytoplasm > cell cortex. Endomembrane system. Cell projection > lamellipodium. Colocalizes with activated RAC3 to endomembranes and at the cell periphery in lamellipodia.
  • Information by UniProt
  • Database links

  • Alternative names

    • BCON3 antibody
    • MADM antibody
    • MUDPNP antibody
    • Multiple domain putative nuclear protein antibody
    • Myeloid leukemia factor 1 adaptor molecule antibody
    • NRBP antibody
    • NRBP_HUMAN antibody
    • NRBP1 antibody
    • Nuclear receptor binding protein antibody
    • Nuclear receptor-binding protein antibody
    see all

Images

  • Anti-MADM antibody (ab154048) at 1/1000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Anti-MADM antibody (ab154048) at 1/10000 dilution + Mouse brain tissue lysate at 50 µg

    Predicted band size: 60 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed A431 cells, labeling MADM with ab154048 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue, labeling MADM with ab154048 at 1/250 dilution.

References

ab154048 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab154048.
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