Anti-MAEA antibody (ab232987)
Key features and details
- Rabbit polyclonal to MAEA
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-MAEA antibody
See all MAEA primary antibodies -
Description
Rabbit polyclonal to MAEA -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Xenopus laevis, Zebrafish, Cynomolgus monkey, Orangutan -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human MAEA aa 100 to the C-terminus. (Expressed in E.coli).
Database link: Q7L5Y9 -
Positive control
- WB: Recombinant human MAEA protein; Mouse cerebrum and pancreas lysates. IHC-P: Human breast cancer, skin cancer, cerebrum, colorectal cancer, bile duct cancer and kidney tissues.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
ab232987 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab232987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 45 kDa.
|
|
IHC-P |
Use a concentration of 5 - 20 µg/ml.
|
Notes |
---|
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 45 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
Target
-
Function
Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 CTLH domain.
Contains 1 LisH domain. -
Developmental stage
Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. -
Cellular localization
Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton. Localized as nuclear speckled-like pattern. - Information by UniProt
-
Database links
- Entrez Gene: 426024 Chicken
- Entrez Gene: 10296 Human
- Entrez Gene: 59003 Mouse
- Entrez Gene: 298982 Rat
- Entrez Gene: 443783 Xenopus laevis
- Entrez Gene: 321575 Zebrafish
- Omim: 606801 Human
- SwissProt: Q5F398 Chicken
see all -
Alternative names
- 1110030D19Rik antibody
- Cell proliferation inducing gene 5 protein antibody
- Cell proliferation-inducing gene 5 protein antibody
see all
Images
-
Anti-MAEA antibody (ab232987) at 2 µg/ml + Recombinant human MAEA protein
Predicted band size: 45 kDa -
Anti-MAEA antibody (ab232987) at 2 µg/ml + Mouse cerebrum lysate
Predicted band size: 45 kDa -
Anti-MAEA antibody (ab232987) at 2 µg/ml + Mouse pancreas lysate
Predicted band size: 45 kDa -
Paraffin-embedded human breast cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human skin cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human cerebrum tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human colorectal cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human kidney tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Paraffin-embedded human bile duct cancer tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
-
Formalin-fixed, paraffin-embedded human stomach tissue stained for MAEA using ab232987 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab232987 has not yet been referenced specifically in any publications.