Anti-MAEA antibody (ab65239)
Key features and details
- Rabbit polyclonal to MAEA
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAEA antibody
See all MAEA primary antibodies -
Description
Rabbit polyclonal to MAEA -
Host species
Rabbit -
Specificity
ab65239 detects endogenous levels of total MAEA protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide derived from an internal sequence of human MAEA.
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Positive control
- COLO205 cell extracts.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab65239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa). |
Target
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Function
Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Contains 1 CTLH domain.
Contains 1 LisH domain. -
Developmental stage
Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis. -
Cellular localization
Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton. Localized as nuclear speckled-like pattern. - Information by UniProt
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Database links
- Entrez Gene: 10296 Human
- Entrez Gene: 59003 Mouse
- Entrez Gene: 298982 Rat
- Omim: 606801 Human
- SwissProt: Q7L5Y9 Human
- SwissProt: Q4VC33 Mouse
- SwissProt: Q5RKJ1 Rat
- Unigene: 139896 Human
see all -
Alternative names
- 1110030D19Rik antibody
- Cell proliferation inducing gene 5 protein antibody
- Cell proliferation-inducing gene 5 protein antibody
see all
Images
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Western blot - Anti-MAEA antibody (ab65239)All lanes : Anti-MAEA antibody (ab65239) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : COLO205 cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 45 kDa
Observed band size: 45 kDa
References (2)
ab65239 has been referenced in 2 publications.
- McTavish CJ et al. Regulation of c-Raf Stability through the CTLH Complex. Int J Mol Sci 20:N/A (2019). PubMed: 30795516
- Mao X et al. Evaluation of erythroblast macrophage protein related to erythroblastic islands in patients with hematopoietic stem cell transplantation. Eur J Med Res 18:9 (2013). WB ; Human . PubMed: 23566571
