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    maea-antibody-ab65239.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-MAEA antibody (ab65239)

  • Datasheet
  • SDS
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Abpromise

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Western blot - Anti-MAEA antibody (ab65239)

    Key features and details

    • Rabbit polyclonal to MAEA
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MAEA antibody
      See all MAEA primary antibodies
    • Description

      Rabbit polyclonal to MAEA
    • Host species

      Rabbit
    • Specificity

      ab65239 detects endogenous levels of total MAEA protein.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse, Rat
    • Immunogen

      Synthetic peptide derived from an internal sequence of human MAEA.

    • Positive control

      • COLO205 cell extracts.
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Stem Cells
      • Hematopoietic Progenitors
      • Myeloid
      • Erythrocytic Lineage

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    Our Abpromise guarantee covers the use of ab65239 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

    Target

    • Function

      Plays a role in erythroblast enucleation and in the development of the mature macrophages. Mediates the attachment of erythroid cell to mature macrophages, in correlation with the presence of MAEA at cell surface of mature macrophages; This MAEA-mediated contact inhibits erythroid cells apoptosis. Participates to erythroblastic island formation, which is the functional unit of definitive erythropoiesis. Associates with F-actin to regulate actin distribution in erythroblasts and macrophages. May contribute to nuclear architecture and cells division events.
    • Tissue specificity

      Ubiquitous.
    • Sequence similarities

      Contains 1 CTLH domain.
      Contains 1 LisH domain.
    • Developmental stage

      Localized with condensed chromatin at prophase; Detected in nuclear spindle poles at metaphase and in the contractile ring during telophase and cytokinesis.
    • Cellular localization

      Nucleus matrix. Cell membrane. Cytoplasm, cytoskeleton. Localized as nuclear speckled-like pattern.
    • Target information above from: UniProt accession Q7L5Y9 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10296 Human
      • Entrez Gene: 59003 Mouse
      • Entrez Gene: 298982 Rat
      • Omim: 606801 Human
      • SwissProt: Q7L5Y9 Human
      • SwissProt: Q4VC33 Mouse
      • SwissProt: Q5RKJ1 Rat
      • Unigene: 139896 Human
      • Unigene: 138617 Mouse
      • Unigene: 281642 Mouse
      • Unigene: 101181 Rat
      see all
    • Alternative names

      • 1110030D19Rik antibody
      • Cell proliferation inducing gene 5 protein antibody
      • Cell proliferation-inducing gene 5 protein antibody
      • EMLP antibody
      • EMP antibody
      • Erythroblast macrophage protein antibody
      • GID complex subunit 9 FYV10 homolog antibody
      • GID9 antibody
      • HLC 10 antibody
      • HLC-10 antibody
      • HLC10 antibody
      • Human lung cancer oncogene 10 protein antibody
      • Lung cancer related protein 10 antibody
      • Macrophage erythroblast attacher antibody
      • MAEA antibody
      • MAEA_HUMAN antibody
      • MGC93683 antibody
      • PIG5 antibody
      • Proliferation inducing gene 5 antibody
      see all

    Images

    • Western blot - Anti-MAEA antibody (ab65239)
      Western blot - Anti-MAEA antibody (ab65239)
      All lanes : Anti-MAEA antibody (ab65239) at 1/500 dilution

      Lane 1 : COLO205 cell extract
      Lane 2 : COLO205 cell extract with immunising peptide at 5 µg

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 45 kDa
      Observed band size: 45 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab65239? Please let us know so that we can cite the reference in this datasheet.

    ab65239 has been referenced in 2 publications.

    • McTavish CJ  et al. Regulation of c-Raf Stability through the CTLH Complex. Int J Mol Sci 20:N/A (2019). PubMed: 30795516
    • Mao X  et al. Evaluation of erythroblast macrophage protein related to erythroblastic islands in patients with hematopoietic stem cell transplantation. Eur J Med Res 18:9 (2013). WB ; Human . PubMed: 23566571

    Customer reviews and Q&As

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