Product nameAnti-Mael antibody
See all Mael primary antibodies
DescriptionRabbit polyclonal to Mael
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse
- This antibody gave a positive result in Mouse Testis Whole Tissue Lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS. pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab28661 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|ICC/IF||1/100. PubMed: 18694567|
FunctionPlays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with piP-bodies suggests a participation in the secondary piRNAs metabolic process. Required for localization of germ-cell factors to the meiotic nuage.
Tissue specificityTestis-specific. Expressed in various cancer cell lines, probably due to demethylation of its promoter.
Sequence similaritiesBelongs to the maelstrom family.
Contains 1 HMG box DNA-binding domain.
Cellular localizationCytoplasm. Nucleus. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Specifically localizes to piP-bodies, a subset of the nuage which contains secondary piRNAs.
- Information by UniProt
- cancer/testis antigen 128 antibody
- CT128 antibody
- FLJ14904 antibody
Anti-Mael antibody (ab28661) at 2 µg/ml + Mouse Testis Whole Tissue Lysate at 20 µg
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Additional bands at: 25 kDa, 37 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Kim SH et al. Mael is essential for cancer cell survival and tumorigenesis through protection of genetic integrity. Oncotarget 8:5026-5037 (2017). WB ; Human . Read more (PubMed: 27926513) »
- Li Q et al. MAEL expression links epithelial-mesenchymal transition and stem cell properties in colorectal cancer. Int J Cancer 139:2502-11 (2016). Read more (PubMed: 27537253) »