Overview

  • Product name
  • Description
    Rabbit polyclonal to MAF1
  • Host species
    Rabbit
  • Specificity
    ab230499 recognizes endogenous levels of MAF1 protein.
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human MAF1 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9H063

  • Positive control
    • IHC-P: Human colon tissue. WB: HEK-293T, Raw264.7 and H9c2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab230499 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100.
WB 1/500 - 1/1000. Predicted molecular weight: 29 kDa.

Target

  • Function
    Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.
  • Sequence similarities
    Belongs to the MAF1 family.
  • Post-translational
    modifications
    Phosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function.
  • Cellular localization
    Nucleus. Cytoplasm.
  • Information by UniProt
  • Database links
  • Alternative names
    • Homolog of yeast MAF1 antibody
    • maf1 antibody
    • MAF1 homolog (S. cerevisiae) antibody
    • MAF1 homolog antibody
    • MAF1_HUMAN antibody
    • MGC20332 antibody
    • MGC31779 antibody
    • MGC39758 antibody
    • Repressor of RNA polymerase III transcription MAF1 homolog antibody
    see all

Images

  • All lanes : Anti-MAF1 antibody (ab230499) at 1/500 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate

    Predicted band size: 29 kDa

  • Formalin-fixed, paraffin-embedded human colon tissue stained for MAF1 using ab230499 at 1/100 dilution in immunohistochemical analysis.

References

ab230499 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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