Key features and details
- Rabbit polyclonal to MAF1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-MAF1 antibody
See all MAF1 primary antibodies
DescriptionRabbit polyclonal to MAF1
Specificityab230499 recognizes endogenous levels of MAF1 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Synthetic peptide within Human MAF1 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9H063
- IHC-P: Human colon tissue. WB: HEK-293T, Raw264.7 and H9c2 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab230499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
FunctionElement of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.
Sequence similaritiesBelongs to the MAF1 family.
modificationsPhosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Homolog of yeast MAF1 antibody
- maf1 antibody
- MAF1 homolog (S. cerevisiae) antibody
All lanes : Anti-MAF1 antibody (ab230499) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate
Predicted band size: 29 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for MAF1 using ab230499 at 1/100 dilution in immunohistochemical analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230499 has not yet been referenced specifically in any publications.