Product nameAnti-MAF1 antibody
See all MAF1 primary antibodies
DescriptionRabbit polyclonal to MAF1
Specificityab230499 recognizes endogenous levels of MAF1 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Synthetic peptide within Human MAF1 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9H063
- IHC-P: Human colon tissue. WB: HEK-293T, Raw264.7 and H9c2 whole cell lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.3
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab230499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 29 kDa.|
FunctionElement of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA.
Sequence similaritiesBelongs to the MAF1 family.
modificationsPhosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function.
Cellular localizationNucleus. Cytoplasm.
- Information by UniProt
- Homolog of yeast MAF1 antibody
- maf1 antibody
- MAF1 homolog (S. cerevisiae) antibody
All lanes : Anti-MAF1 antibody (ab230499) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate
Predicted band size: 29 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for MAF1 using ab230499 at 1/100 dilution in immunohistochemical analysis.
ab230499 has not yet been referenced specifically in any publications.