Anti-MAF1 antibody (ab230499)
Key features and details
- Rabbit polyclonal to MAF1
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MAF1 antibody
See all MAF1 primary antibodies -
Description
Rabbit polyclonal to MAF1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide within Human MAF1 (internal sequence) conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9H063 -
Positive control
- IHC-P: Human colon tissue. WB: HEK-293T, Raw264.7 and H9c2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230499 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 29 kDa.
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Notes |
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IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 29 kDa. |
Target
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Function
Element of the mTORC1 signaling pathway that acts as a mediator of diverse signals and that represses RNA polymerase III transcription. Inhibits the de novo assembly of TFIIIB onto DNA. -
Sequence similarities
Belongs to the MAF1 family. -
Post-translational
modificationsPhosphorylated at Ser-60, Ser-68 and Ser-75; the major sites of phosphorylation. Nuclear accumulation correlates with a concomitant dephosphorylation. Phosphorylation may attenuate its RNA polymerase III-repressive function. -
Cellular localization
Nucleus. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 84232 Human
- Entrez Gene: 68877 Mouse
- Entrez Gene: 315093 Rat
- Omim: 610210 Human
- SwissProt: Q9H063 Human
- SwissProt: Q9D0U6 Mouse
- SwissProt: Q5XIH0 Rat
- Unigene: 19673 Human
see all -
Alternative names
- Homolog of yeast MAF1 antibody
- maf1 antibody
- MAF1 homolog (S. cerevisiae) antibody
see all
Images
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All lanes : Anti-MAF1 antibody (ab230499) at 1/500 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : Raw264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : H9c2 (Rat heart myoblast cell line) whole cell lysate
Predicted band size: 29 kDa -
Formalin-fixed, paraffin-embedded human colon tissue stained for MAF1 using ab230499 at 1/100 dilution in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230499 has been referenced in 1 publication.
- Chen S et al. Maf1 Ameliorates Sepsis-Associated Encephalopathy by Suppressing the NF-kB/NLRP3 Inflammasome Signaling Pathway. Front Immunol 11:594071 (2020). PubMed: 33424842