Product nameAnti-MafA antibody
See all MafA primary antibodies
DescriptionRabbit polyclonal to MafA
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
- This antibody gave a positive signal in human brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab26405 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 37 kDa).
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data), however publications have shown the human protein to be detected at 47 kDa according to reference PMID 12011435, http://www.pnas.org/cgi/content/full/99/10/6737. Our data shows ab26405 to detect a band at 36 kDa in human brain, kidney and liver which is similar to that seen in other publications (PMID:14680841, PMID:18159220 (expression profile) ). The mouse protein has also been observed at 47 kDa according to reference PMID 12368292.
|IHC-P||Use a concentration of 0.8 - 4 µg/ml.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
modificationsUbiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
- Information by UniProt
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
The image shows human pancreas tissue in which some cells exhibit nuclear staining. The antigen retrieval method used was Tris EDTA pH9.
ab26405 staining MAFA in Human PANC-1 cell line transfected with synthetic mRNA by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.05% Triton X-100 and blocked with 5% serum for 30 minutes at 20°C. Samples were incubated with primary antibody (1/200) for 30 minutes at 37°C. An Alexa Fluor® 555-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
Anti-MafA antibody (ab26405) at 1 µg/ml + Brain (Human) Tissue Lysate at 20 µg
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 37 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Human MAFA has a predicted molecular weight of 37 kDa (Swiss-Prot data). Some publications suggest that the human protein should be detected at 47 kDa (PMID 12011435). Our data shows that ab26405 detects a band at 36 kDa in human brain, which is similar to that seen in other publications [PMID:14680841, PMID:18159220 (expression profile)]. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This product has been referenced in:
- Axelsson AS et al. Sox5 regulates beta-cell phenotype and is reduced in type 2 diabetes. Nat Commun 8:15652 (2017). WB, IHC-P . Read more (PubMed: 28585545) »
- Wang P et al. A high-throughput chemical screen reveals that harmine-mediated inhibition of DYRK1A increases human pancreatic beta cell replication. Nat Med 21:383-8 (2015). WB, ICC/IF . Read more (PubMed: 25751815) »