Key features and details
- Rabbit polyclonal to MAFA
- Suitable for: IHC-P
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-MAFA antibody
See all MafA primary antibodies
DescriptionRabbit polyclonal to MAFA
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse
Synthetic peptide, corresponding to a region between C terminal amino acids 300-359 of Mouse MafA (NP_919331.1).
- Mouse Pancreas tissue.
Concentration is optimized for IHC and not determined
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab85008 was affinity purified using the peptide immobilized on solid support.
Our Abpromise guarantee covers the use of ab85008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Likely to work with frozen sections.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a transcriptional factor. Specifically binds the insulin enhancer element RIPE3b and activates insulin gene expression. Cooperates synergistically with NEUROD1 and PDX1. Phosphorylation by GSK3 increases its transcriptional activity and is required for its oncogenic activity. Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
modificationsUbiquitinated, leading to its degradation by the proteasome.
Ser-14 and Ser-65 appear to be the major phosphorylation sites. Phosphorylated by MAPK13 on serine and threonine residues (Probable). Phosphorylation by GSK3 requires prior phosphorylation of Ser-65 by another kinase. Phosphorylation proceeds then from Ser-61 to Thr-57, Thr-53 and Ser-49. GSK3-mediated phosphorylation increases its transcriptional activity through the recruitment of the coactivator PCAF, is required for its transforming activity and leads to its degradation through an ubiquitin/proteasome-dependent pathway.
Cellular localizationNucleus. Detected in nuclei of pancreas islet beta cells.
- Information by UniProt
- HMafA antibody
- Mafa antibody
- MAFA_HUMAN antibody
ab85008 has not yet been referenced specifically in any publications.