Product nameAnti-MafB antibody
See all MafB primary antibodies
DescriptionRabbit polyclonal to MafB
Tested applicationsSuitable for: ELISA, WBmore details
Species reactivityReacts with: Mouse, Human
3 synthetic peptides (human) conjugated to KLH
- Purchase matching WB positive control:Recombinant Human MafB protein
- Induced culture of E.coli (one shot Top10 competent cells). Plasmid name: pBAD-DEST49.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Concentration information loading...
PurityImmunogen affinity purified
Purification notesCrude serum, final bleed.
Our Abpromise guarantee covers the use of ab82837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionActs as a transcriptional activator or repressor. Plays a pivotal role in regulating lineage-specific hematopoiesis by repressing ETS1-mediated transcription of erythroid-specific genes in myeloid cells. Required for monocytic, macrophage, podocyte and islet beta cell differentiation. Involved in renal tubule survival and F4/80 maturation. Activates the insulin and glucagon promoters. Together with PAX6, transactivates weakly the glucagon gene promoter through the G1 element. SUMO modification controls its transcriptional activity and ability to specify macrophage fate. Binds element G1 on the glucagon promoter (By similarity). Involved either as an oncogene or as a tumor suppressor, depending on the cell context.
Involvement in diseaseDefects in MAFB are the cause of multicentric carpotarsal osteolysis syndrome (MCTO) [MIM:166300]. MCTO is a rare skeletal disorder, usually presenting in early childhood with a clinical picture mimicking juvenile rheumatoid arthritis. Progressive destruction of the carpal and tarsal bone usually occurs and other bones may also be involved. Chronic renal failure is a frequent component of the syndrome. Mental retardation and minor facial anomalies have been noted in some patients.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
DomainThe leucine-zipper domain is involved in the interaction with LRPICD.
modificationsPhosphorylated by GSK3 and MAPK13 on serine and threonine residues.
Sumoylated. Sumoylation on Lys-32 and Lys-297 stimulates its transcriptional repression activity and promotes macrophage differentiation from myeloid progenitors.
- Information by UniProt
- Kreisler antibody
- Kreisler (mouse) maf related leucine zipper homolog antibody
- Kreisler maf related leucine zipper homolog antibody
All lanes : Anti-MafB antibody (ab82837) at 1/500 dilution
Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
Lane 2 : induced culture of E.coli (one shot Top10 competent cells)
All lanes : anti-Rabbit IgG HRP at 1/1000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Predicted molecular weight is 35.7kDa, tag is 14kDa.
ab82837 has not yet been referenced specifically in any publications.