Anti-MAFG antibody (ab168453)
Key features and details
- Mouse polyclonal to MAFG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MAFG antibody
See all MAFG primary antibodies -
Description
Mouse polyclonal to MAFG -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow -
Immunogen
Recombinant full length protein corresponding to amino acids 1-162 of Human MAFG. (NP_002350.1)
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Positive control
- Transfected 293T cell lysate; Daoy cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab168453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent dilution. Detects a band of approximately 18 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use at an assay dependent dilution. Detects a band of approximately 18 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+). -
Tissue specificity
Highly expressed in skeletal muscle. Also expressed in heart and brain. -
Sequence similarities
Belongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain. -
Post-translational
modificationsAcetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.
Sumoylation at Lys-14 is required for active transcriptional repression. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 769355 Chicken
- Entrez Gene: 515219 Cow
- Entrez Gene: 4097 Human
- Entrez Gene: 17134 Mouse
- Entrez Gene: 64188 Rat
- Omim: 602020 Human
- SwissProt: Q90889 Chicken
- SwissProt: A5PJV0 Cow
see all -
Alternative names
- AA545192 antibody
- Basic leucine zipper transcription factor MafG antibody
- C630022N07Rik antibody
see all
Images
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All lanes : Anti-MAFG antibody (ab168453) at 1 µg/ml
Lane 1 : MAGF transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat Anti-mouse HRP at 1/2500 dilution -
Immunocytochemistry/Immunofluorescence of Daoy cells labeling MAFG with ab168453 at 10µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab168453 has not yet been referenced specifically in any publications.