Key features and details
- Mouse polyclonal to MAFG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAFG antibody
See all MAFG primary antibodies
DescriptionMouse polyclonal to MAFG
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Cow
- Transfected 293T cell lysate; Daoy cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Constituent: 100% PBS
Concentration information loading...
PurityProtein A purified
- Epigenetics and Nuclear Signaling
- Polymerase associated factors
- Pol II Transcription
Our Abpromise guarantee covers the use of ab168453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution. Detects a band of approximately 18 kDa.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionSince they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor. Transcription factor, component of erythroid-specific transcription factor NF-E2. Activates globin gene expression when associated with NF-E2. May be involved in signal transduction of extracellular H(+).
Tissue specificityHighly expressed in skeletal muscle. Also expressed in heart and brain.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
modificationsAcetylated in erythroid cells by CREB-binding protein (CBP). Acetylation augments the DNA-binding activity of NFE2, but has no effect on binding NFE2.
Sumoylation at Lys-14 is required for active transcriptional repression.
- Information by UniProt
- AA545192 antibody
- Basic leucine zipper transcription factor MafG antibody
- C630022N07Rik antibody
All lanes : Anti-MAFG antibody (ab168453) at 1 µg/ml
Lane 1 : MAGF transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 15 µl per lane.
All lanes : Goat Anti-mouse HRP at 1/2500 dilution
Immunocytochemistry/Immunofluorescence of Daoy cells labeling MAFG with ab168453 at 10µg/ml.
ab168453 has not yet been referenced specifically in any publications.