Key features and details
- Rabbit polyclonal to MAFK
- Suitable for: IP, ChIP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAFK antibody
See all MAFK primary antibodies
DescriptionRabbit polyclonal to MAFK
Tested applicationsSuitable for: IP, ChIP, WBmore details
Species reactivityReacts with: Human
- WB: Jurkat whole cell lysate. IP: Jurkat whole cell lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229766 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||1/100 - 1/500.|
|ChIP||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Predicted molecular weight: 18 kDa.|
FunctionSince they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Erythroid transcription factor NF E2 p18 subunit antibody
- Erythroid transcription factor NF-E2 p18 subunit antibody
- FLJ32205 antibody
Anti-MAFK antibody (ab229766) at 1/500 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Predicted band size: 18 kDa
15% SDS-PAGE gel.
MAFK was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with 4 μg of ab229766. Western blot was performed from the immunoprecipitate using ab229766 at 1/500 dilution.
Lane 1: Control IgG instead of ab229766 in Jurkat whole cel lysate (input).
Lane 2: ab229766 IP in Jurkat whole cell lysate.
Cross-linked ChIP was performed with HepG2 (human liver hepatocellular carcinoma cell line) chromatin extract treated with LPS (10 ng/ml for 1 h) using 5 μg of either normal rabbit IgG or ab229766.
The precipitated DNA was detected by PCR with primer set targeting to IL-8 or GAPDH.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229766 has not yet been referenced specifically in any publications.