Overview

  • Product name

  • Description

    Rabbit polyclonal to MAFK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ChIP, IP, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Zebrafish
  • Immunogen

    Synthetic peptide:

    TTNPKPNKALKVKKEA

    conjugated to KLH by a C-terminal Cysteine residue linker, corresponding to amino acids 2-17 of Human MAFK

  • Positive control

    • Whole extracts of the A431 cell line

Properties

Applications

Our Abpromise guarantee covers the use of ab50322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent concentration. PubMed: 23291531
IP Use 4µl for 108 cells.

Use 4 - 8 µl immunoprecipitates MAFF from the A431 cell line.

WB 1/4000 - 1/8000. Predicted molecular weight: 17 kDa.

Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.

ICC/IF Use at an assay dependent concentration. PubMed: 24465428

Target

  • Function

    Since they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
  • Sequence similarities

    Belongs to the bZIP family. Maf subfamily.
    Contains 1 bZIP domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Erythroid transcription factor NF E2 p18 subunit antibody
    • Erythroid transcription factor NF-E2 p18 subunit antibody
    • FLJ32205 antibody
    • MAFK antibody
    • MAFK_HUMAN antibody
    • MGC71717 antibody
    • Nfe2u antibody
    • P18 antibody
    • Transcription factor MafK antibody
    • V maf musculoaponeurotic fibrosarcoma oncogene homolog K (avian) antibody
    see all

Images

  • ChIP analysis using ab50322 binding MAFK in Human primary macrophage. Cells were cross-linked with formaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. Protein binding was detected using real-time PCR.

     

    See Abreview

  • Lanes 1 & 3 : Anti-MAFK antibody (ab50322) at 1/4000 dilution
    Lane 2 : Anti-MAFK antibody (ab50322) at 1/8000 dilution
    Lane 4 : no primary antibody

    Lanes 1-2 & 4 : A431 whole cell lysate
    Lane 3 : A431 whole cell lysate with MAFF immunizing peptide at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate

    Predicted band size: 17 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?

References

This product has been referenced in:

  • Johnson GS  et al. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. Mol Nutr Food Res 61:N/A (2017). Read more (PubMed: 27860235) »
  • Zheng S  et al. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol 194:1928-37 (2015). Read more (PubMed: 25567680) »
See all 7 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (macrophage)
Specification
macrophage
Treatment
0.1 ug/ml LPS 15 min
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Dec 24 2014

Application
ChIP
Detection step
Real-time PCR
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde

Abcam user community

Verified customer

Submitted Jan 18 2014

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