Product nameAnti-MAFK antibody
See all MAFK primary antibodies
DescriptionRabbit polyclonal to MAFK
Tested applicationsSuitable for: ChIP, IP, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
- Whole extracts of the A431 cell line
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
ChIP Related Products
Our Abpromise guarantee covers the use of ab50322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ChIP||Use at an assay dependent concentration. PubMed: 23291531|
|IP||Use 4µl for 108 cells.
Use 4 - 8 µl immunoprecipitates MAFF from the A431 cell line.
|WB||1/4000 - 1/8000. Predicted molecular weight: 17 kDa.
Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.
|ICC/IF||Use at an assay dependent concentration. PubMed: 24465428|
FunctionSince they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
- Information by UniProt
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ChIP analysis using ab50322 binding MAFK in Human primary macrophage. Cells were cross-linked with formaldehyde. Samples were incubated with primary antibody (1/500) for 12 hours at 4°C. Protein binding was detected using real-time PCR.
Lanes 1 & 3 : Anti-MAFK antibody (ab50322) at 1/4000 dilution
Lane 2 : Anti-MAFK antibody (ab50322) at 1/8000 dilution
Lane 4 : no primary antibody
Lanes 1-2 & 4 : A431 whole cell lysate
Lane 3 : A431 whole cell lysate with MAFF immunizing peptide at 10 µg
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 17 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Johnson GS et al. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. Mol Nutr Food Res 61:N/A (2017). Read more (PubMed: 27860235) »
- Zheng S et al. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol 194:1928-37 (2015). Read more (PubMed: 25567680) »