Key features and details
- Rabbit polyclonal to MAFK
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAFK antibody
See all MAFK primary antibodies
DescriptionRabbit polyclonal to MAFK
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Zebrafish
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityIon Exchange Chromatography
ChIP Related Products
Our Abpromise guarantee covers the use of ab50322 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use 4µl for 108 cells.
Use 4 - 8 µl immunoprecipitates MAFF from the A431 cell line.
|WB||1/4000 - 1/8000. Predicted molecular weight: 17 kDa.
Dilute the antibody in PBS containing 5.0% non-fat dry milk and 0.05 % Tween 20. Staining of the MAFF band in immunoblotting is specifically inhibited by the immunizing peptide.
FunctionSince they lack a putative transactivation domain, the small Mafs behave as transcriptional repressors when they dimerize among themselves. However, they seem to serve as transcriptional activators by dimerizing with other (usually larger) basic-zipper proteins and recruiting them to specific DNA-binding sites. Small Maf proteins heterodimerize with Fos and may act as competitive repressors of the NF-E2 transcription factor.
Sequence similaritiesBelongs to the bZIP family. Maf subfamily.
Contains 1 bZIP domain.
- Information by UniProt
- Erythroid transcription factor NF E2 p18 subunit antibody
- Erythroid transcription factor NF-E2 p18 subunit antibody
- FLJ32205 antibody
Lanes 1 & 3 : Anti-MAFK antibody (ab50322) at 1/4000 dilution
Lane 2 : Anti-MAFK antibody (ab50322) at 1/8000 dilution
Lane 4 : no primary antibody
Lanes 1-2 & 4 : A431 whole cell lysate
Lane 3 : A431 whole cell lysate with MAFF immunizing peptide at 10 µg
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 17 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
ab50322 has been referenced in 7 publications.
- Johnson GS et al. A functional pseudogene, NMRAL2P, is regulated by Nrf2 and serves as a coactivator of NQO1 in sulforaphane-treated colon cancer cells. Mol Nutr Food Res 61:N/A (2017). PubMed: 27860235
- Zheng S et al. TAM receptor-dependent regulation of SOCS3 and MAPKs contributes to proinflammatory cytokine downregulation following chronic NOD2 stimulation of human macrophages. J Immunol 194:1928-37 (2015). PubMed: 25567680
- Zhang Z et al. Oxidative stress regulates CFTR gene expression in human airway epithelial cells through a distal antioxidant response element. Am J Respir Cell Mol Biol 52:387-96 (2015). PubMed: 25259561
- Marinov GK et al. Evidence for site-specific occupancy of the mitochondrial genome by nuclear transcription factors. PLoS One 9:e84713 (2014). ICC/IF ; Human . PubMed: 24465428
- Hwang YJ et al. Oleanolic acid regulates NF-?B signaling by suppressing MafK expression in RAW 264.7 cells. BMB Rep 47:524-9 (2014). PubMed: 25059280
- Jolma A et al. DNA-binding specificities of human transcription factors. Cell 152:327-39 (2013). PubMed: 23332764
- Yang D et al. Identification of KAP-1-associated complexes negatively regulating the Ey and ß-major globin genes in the ß-globin locus. J Proteomics 80C:132-144 (2013). ChIP . PubMed: 23291531