Overview

  • Product name
    Anti-MAG antibody [EP971Y]
    See all MAG primary antibodies
  • Description
    Rabbit monoclonal [EP971Y] to MAG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the N-terminus of human MAG.

  • Positive control
    • Rat brain lysate PC-12 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab46803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).
IP 1/50.
Flow Cyt 1/20.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for ICC/IF or IHC-P.
  • Target

    • Function
      Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
      Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • GMA antibody
      • MAG antibody
      • MAG_HUMAN antibody
      • Myelin associated glycoprotein antibody
      • Myelin-associated glycoprotein antibody
      • S MAG antibody
      • S-MAG antibody
      • Sialic acid binding Ig like lectin 4A antibody
      • Sialic acid binding immunoglobulin like lectin 4A antibody
      • Siglec 4a antibody
      • Siglec-4a antibody
      • SIGLEC4A antibody
      • SPG75 antibody
      see all

    Images

    • Anti-MAG antibody [EP971Y] (ab46803) at 1/5000 dilution + Rat brain lysate at 10ug/lane

      Secondary
      Goat-anti rabbit, labelled with HRP. at 1/2000 dilution

      Predicted band size: 69 kDa
      Observed band size: 100 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 22 kDa (possible non-specific binding)

    References

    This product has been referenced in:
    • Zou S  et al. Oligodendrocytes Are Targets of HIV-1 Tat: NMDA and AMPA Receptor-Mediated Effects on Survival and Development. J Neurosci 35:11384-98 (2015). Read more (PubMed: 26269645) »
    See 1 Publication for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 09 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Brainstem)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Brainstem
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 09 2018

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Spinal cord)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Spinal cord
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde

    Mr. Carl Hobbs

    Verified customer

    Submitted Aug 09 2018

    Answer

    DISCOUNT CODE: 100%ABR-EXXXXX
    Expiration date: 27/01/2013
    I am very pleased to hear you would like to accept our offer and test ab46803 in IHC-PFA fixed mouse brain. This code will give you: 1 free PRIMARY ANTIBODY/PROTEIN OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for IHC-PFA fixed mouse brain and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
    For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

    Read More

    Answer

    Thank you for your enquiry and your interest in our products.
    I can certainly offer you a testing discount but we would need to clarify a few things regarding the experiments.
    Could you please confirm if the application will be ICC/IF (Immunocytochemistry) or IHC-P or IHC-Fr (Immunohistochemistry)? Would you use cultured cells or tissue section? What fixative will be applied (ethanol, methanol, acetone, formalin etc)?
    I would advise you to visit the following site where the Terms and Conditions of our testing initiative can be found at: www.abcam.com/collaborationdiscount.
    I look forward to hearing from you soon.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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