Recombinant Anti-MAG/GMA antibody [EP971Y] (ab46803)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP971Y] to MAG/GMA
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
-
Product name
Anti-MAG/GMA antibody [EP971Y]
See all MAG/GMA primary antibodies -
Description
Rabbit monoclonal [EP971Y] to MAG/GMA -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human MAG/GMA aa 50-150.
-
Positive control
- WB: Human, Mouse and Rat brain lysates.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP971Y -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab46803 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa).
|
Notes |
---|
WB
1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 69 kDa). |
Target
-
Function
Adhesion molecule in postnatal neural development that mediates sialic-acid dependent cell-cell interactions between neuronal and myelinating cells. Preferentially binds to alpha-2,3-linked sialic acid. -
Sequence similarities
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 4 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 4099 Human
- Entrez Gene: 17136 Mouse
- Entrez Gene: 29409 Rat
- Omim: 159460 Human
- SwissProt: P20916 Human
- SwissProt: P20917 Mouse
- SwissProt: P07722 Rat
- Unigene: 643440 Human
see all -
Alternative names
- GMA antibody
- MAG antibody
- MAG_HUMAN antibody
see all
Images
-
All lanes : Anti-MAG/GMA antibody [EP971Y] (ab46803) at 1/10000 dilution
Lane 1 : Human brain lysate
Lane 2 : Mouse brain lysate
Lane 3 : Rat brain lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 69 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?Blocking and diluting dilution: 5% NFDM/TBST
Exposure time:
Lane1: 180 seconds
Lane2: 80 seconds
Lane3: 60 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (1)
ab46803 has been referenced in 1 publication.
- Zou S et al. Oligodendrocytes Are Targets of HIV-1 Tat: NMDA and AMPA Receptor-Mediated Effects on Survival and Development. J Neurosci 35:11384-98 (2015). PubMed: 26269645