Overview

  • Product name

    Anti-MAGE3 antibody - C-terminal
    See all MAGE3 primary antibodies
  • Description

    Rabbit polyclonal to MAGE3 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human MAGE3 (C terminal). NP_005353.
    Database link: P43357

  • Positive control

    • Human testis tissue, A549 and PC3 cell lysates. Human tonsil tissue.
  • General notes

     This product was previously labelled as MAGEA3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab199152 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 35 kDa.

Target

  • Function

    Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen MZ2 D antibody
    • Antigen MZ2-D antibody
    • Cancer/testis antigen 1.3 antibody
    • Cancer/testis antigen family 1 member 3 antibody
    • CT1.3 antibody
    • HIP8 antibody
    • HYPD antibody
    • MAGA3_HUMAN antibody
    • MAGE 3 antigen antibody
    • MAGE family member A3 antibody
    • MAGE-3 antigen antibody
    • MAGE3 antibody
    • MAGEA3 antibody
    • MAGEA6 antibody
    • Melanoma antigen family A 3 antibody
    • Melanoma associated antigen 3 antibody
    • Melanoma-associated antigen 3 antibody
    • MGC1461 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MAGE3 with ab199152 at 1/50 dilution.

  • All lanes : Anti-MAGE3 antibody - C-terminal (ab199152) at 1/400 dilution

    Lane 1 : Human testis tissue lysate
    Lane 2 : A549 lysate
    Lane 3 : PC3 lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 35 kDa


    Exposure time: 2 minutes


    Gel: 10% SDS-PAGE

References

ab199152 has not yet been referenced specifically in any publications.

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