Key features and details
- Rabbit polyclonal to MAGE3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MAGE3 antibody - C-terminal
See all MAGE3 primary antibodies
DescriptionRabbit polyclonal to MAGE3 - C-terminal
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human MAGE3 (C terminal). NP_005353.
Database link: P43357
- Human testis tissue, A549 and PC3 cell lysates. Human tonsil tissue.
This product was previously labelled as MAGEA3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab199152 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 35 kDa.|
FunctionProposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
Sequence similaritiesContains 1 MAGE domain.
- Information by UniProt
- Antigen MZ2 D antibody
- Antigen MZ2-D antibody
- Cancer/testis antigen 1.3 antibody
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling MAGE3 with ab199152 at 1/50 dilution.
All lanes : Anti-MAGE3 antibody - C-terminal (ab199152) at 1/400 dilution
Lane 1 : Human testis tissue lysate
Lane 2 : A549 lysate
Lane 3 : PC3 lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 35 kDa
Exposure time: 2 minutes
Gel: 10% SDS-PAGE
ab199152 has not yet been referenced specifically in any publications.