Recombinant
RabMAb

Recombinant Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)

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Overview

  • Product name

    Anti-MAGE3 antibody [EPR20658] - BSA and Azide free
    See all MAGE3 primary antibodies

  • Description

    Rabbit monoclonal [EPR20658] to MAGE3 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody shows cross reactivity to MAGEA2 and MAGEA6 as well as weak cross reactivity to MAGEA1 and MAGEA12. It does not cross react with MAGEA4 and MAGEA8.

  • Tested applications

    Suitable for: ICC/IF, IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human MAGE3 aa 50 to the C-terminus. The exact sequence is proprietary.
    Database link: P43357

  • Positive control

    • ICC/IF: A-375 cells.

  • General notes

    ab228521 is the carrier-free version of ab213722 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab228521 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MAGEA3

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab228521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 45-50 kDa (predicted molecular weight: 35 kDa).

Target

  • Function

    Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.

  • Sequence similarities

    Contains 1 MAGE domain.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen MZ2 D antibody
      • Antigen MZ2-D antibody
      • Cancer/testis antigen 1.3 antibody
      • Cancer/testis antigen family 1 member 3 antibody
      • CT1.3 antibody
      • HIP8 antibody
      • HYPD antibody
      • MAGA3_HUMAN antibody
      • MAGE 3 antigen antibody
      • MAGE family member A3 antibody
      • MAGE-3 antigen antibody
      • MAGE3 antibody
      • MAGEA3 antibody
      • MAGEA6 antibody
      • Melanoma antigen family A 3 antibody
      • Melanoma associated antigen 3 antibody
      • Melanoma-associated antigen 3 antibody
      • MGC1461 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)
      Immunocytochemistry/ Immunofluorescence - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling MAGE3 with ab213722 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining in HeLa cell line (PMID: 23946777, PMID: 23390371) is observed.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213722).

    • Immunoprecipitation - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)
      Immunoprecipitation - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)

      MAGE3 was immunoprecipitated from 0.35 mg of A-375 (human malignant melanoma cell line) whole cell lysate with ab213722 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab213722 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: A-375 whole cell lysate 10 μg (Input).
      Lane 2: ab213722 IP in A-375 whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab213722 in A-375 whole cell lysate.

      Exposure time: 1 second.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213722).

    • Immunocytochemistry/ Immunofluorescence - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)
      Immunocytochemistry/ Immunofluorescence - Anti-MAGE3 antibody [EPR20658] - BSA and Azide free (ab228521)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A-375 (human malignant melanoma cell line) cells labeling MAGE3 with ab213722 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear and cytoplasmic staining in A-375 cell line (PMID: 23946777, PMID: 23390371) is observed.

      The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab213722).

    References

    ab228521 has not yet been referenced specifically in any publications.

    Publishing research using ab228521? Please let us know so that we can cite the reference in this datasheet.

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