Key features and details
- Mouse monoclonal [OTI1H1] to MAGE3
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MAGE3 antibody [OTI1H1]
See all MAGE3 primary antibodies
DescriptionMouse monoclonal [OTI1H1] to MAGE3
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MAGE3 aa 1-314.
MPLEQRSQHCKPEEGLEARGEALGLVGAQAPATEEQEAASSSSTLVEVTL GEVPAAESPDPPQSPQGASSLPTTMNYPLWSQSYEDSSNQEEEGPSTFPD LESEFQAALSRKVAELVHFLLLKYRAREPVTKAEMLGSVVGNWQYFFPVI FSKASSSLQLVFGIELMEVDPIGHLYIFATCLGLSYDGLLGDNQIMPKAG LLIIVLAIIAREGDCAPEEKIWEELSVLEVFEGREDSILGDPKKLLTQHF VQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHMVKISGGPHIS YPPLHEWVLREGEE
Database link: NP_005353
- HEK293T cell lysate transfected with pCMV6-ENTRY MAGE3 cDNA; Human kidney and colon adenocarcinoma tissues
The clone number has been updated from 1H1 to OTI1H1, both clone numbers name the same clone.
Dilute in PBS (pH7.3) before use.
This product was changed from ascites to tissue culture supernatant on 10th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
This product was previously labelled as MAGEA3
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 48% PBS, 50% Glycerol, 1% BSA
Concentration information loading...
PurityProtein G purified
Purification notesab140678 is purified from TCS by affinity chromatography.
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab140678 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 35 kDa.
1/2000. Predicted molecular weight: 35 kDa.
FunctionProposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes and placenta. Never expressed in kidney tumors, Leukemias and lymphomas.
Sequence similaritiesContains 1 MAGE domain.
- Information by UniProt
- Antigen MZ2 D antibody
- Antigen MZ2-D antibody
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All lanes : Anti-MAGE3 antibody [OTI1H1] (ab140678) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY MAGE3 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 35 kDa
HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC210002)
Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labelling MAGE3 with ab140678 at 1/150 dilution.
ab140678 has been referenced in 2 publications.
- Wu F et al. miR-1273g silences MAGEA3/6 to inhibit human colorectal cancer cell growth via activation of AMPK signaling. Cancer Lett 435:1-9 (2018). PubMed: 30056111
- Chen X et al. Expression and prognostic relevance of MAGE-A3 and MAGE-C2 in non-small cell lung cancer. Oncol Lett 13:1609-1618 (2017). IHC-P ; Human . PubMed: 28454298