Product nameAnti-MAGEA1 antibody [EPR4276]
See all MAGEA1 primary antibodies
DescriptionRabbit monoclonal [EPR4276] to MAGEA1
SpecificityBased on the sequence blast, the immunogen of ab109108 has 85.7% - 92.3% homology with the family members MAGEB3, MAGE2, MAGE3, MAGE6, MAGE9, MAGE10 and MAGE12
Tested applicationsSuitable for: WB, Flow Cyt, ICC/IFmore details
Unsuitable for: IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human MAGEA1 aa 200-300. The exact sequence is proprietary.
- WB: A375, HeLa, Jurkat, HepG2, and MCF7 lysates. Flow Cyt: HeLa cells. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab109108 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 46 kDa (predicted molecular weight: 34 kDa).|
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
Tissue specificityExpressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.
Sequence similaritiesContains 1 MAGE domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- Antigen MZ2 E antibody
- Antigen MZ2-E antibody
- Cancer/testis antigen 1.1 antibody
All lanes : Anti-MAGEA1 antibody [EPR4276] (ab109108) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : MCF7 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 34 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling MAGEA1 with ab109108 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
The negative control is the secondary antibody only.
Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cell line labeling MAGEA1 with ab109108 at 1/500 (red) compared with a Rabbit monoclonal IgG (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077), at 1/2000 dilution was used as the secondary antibody.
ab109108 has not yet been referenced specifically in any publications.