Overview

  • Product name

    Anti-MAGEA1 antibody [SP188]
    See all MAGEA1 primary antibodies
  • Description

    Rabbit monoclonal [SP188] to MAGEA1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MAGEA1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P43355

  • Positive control

    • IHC-P: Human testis, and skin squamous cell carcinoma tissue; WB: HeLa whole cell lysate (ab150035); FC: A431 and SK-OV-3 cells.
  • General notes

     This product was previously labelled as MAGE 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab227664 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 34 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/400.

Target

  • Function

    May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.
  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.
  • Sequence similarities

    Contains 1 MAGE domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Antigen MZ2 E antibody
    • Antigen MZ2-E antibody
    • Cancer/testis antigen 1.1 antibody
    • cancer/testis antigen family 1, member 1 antibody
    • CT1.1 antibody
    • MAGA1_HUMAN antibody
    • MAGE 1 antigen antibody
    • MAGE-1 antigen antibody
    • MAGE1 antibody
    • MAGE1A antibody
    • MAGEA1 antibody
    • Melanoma antigen 1 antibody
    • Melanoma antigen family A 1 (directs expression of antigen MZ2 E) antibody
    • Melanoma antigen family A 1 antibody
    • Melanoma antigen MAGE 1 antibody
    • Melanoma associated antigen 1 antibody
    • Melanoma associated antigen MZ2 E antibody
    • Melanoma-associated antigen 1 antibody
    • MGC9326 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling MAGEA1 with ab227664 at 1/100 dilution (1.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purifiedab227664 at 1:20 dilution (7.25 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

  • Formalin-fixed, paraffin-embedded human testis tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.

  • Anti-MAGEA1 antibody [SP188] (ab227664) at 1/400 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate

    Predicted band size: 34 kDa

  • Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling MAGEA1 with ab227664 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

  • Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.

References

ab227664 has not yet been referenced specifically in any publications.

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