RecombinantRabMAb

Recombinant Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
  • Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
  • Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
  • Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP188] to MAGEA1 - BSA and Azide free
  • Suitable for: WB, Flow Cyt, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-MAGEA1 antibody [SP188] - BSA and Azide free
    See all MAGEA1 primary antibodies

  • Description

    Rabbit monoclonal [SP188] to MAGEA1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MAGEA1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P43355

  • Positive control

    • IHC-P: Human testis, and skin squamous cell carcinoma tissue; Flow Cyt: A431 and SK-OV-3 cells.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    ab243935 is the carrier-free version of ab227664 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab243935 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as MAGE 1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab243935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 34 kDa.
Flow Cyt Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.

  • Sequence similarities

    Contains 1 MAGE domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen MZ2 E antibody
      • Antigen MZ2-E antibody
      • Cancer/testis antigen 1.1 antibody
      • cancer/testis antigen family 1, member 1 antibody
      • CT1.1 antibody
      • MAGA1_HUMAN antibody
      • MAGE 1 antigen antibody
      • MAGE-1 antigen antibody
      • MAGE1 antibody
      • MAGE1A antibody
      • MAGEA1 antibody
      • Melanoma antigen 1 antibody
      • Melanoma antigen family A 1 (directs expression of antigen MZ2 E) antibody
      • Melanoma antigen family A 1 antibody
      • Melanoma antigen MAGE 1 antibody
      • Melanoma associated antigen 1 antibody
      • Melanoma associated antigen MZ2 E antibody
      • Melanoma-associated antigen 1 antibody
      • MGC9326 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human testis tissue sections labeling MAGEA1 with ab227664 at 1/100 dilution (1.45 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227664)
    • Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

      Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purifiedab227664 at 1:20 dilution (7.25 µg/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor© 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227664)

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

      Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227664).

    • Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Flow Cytometry - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

      Flow cytometric analysis of A431 (human epidermoid carcinoma cell line) cell line labeling MAGEA1 with ab227664 at 1/400 dilution (green) compared with a Rabbit IgG negative control (blue).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227664).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

      Formalin-fixed, paraffin-embedded human testis tissue stained for MAGEA1 using ab227664 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab227664).

    • Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)
      Anti-MAGEA1 antibody [SP188] - BSA and Azide free (ab243935)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab243935? Please let us know so that we can cite the reference in this datasheet.

    ab243935 has been referenced in 1 publication.

    • Mao Y  et al. MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells. J Hematol Oncol 12:106 (2019). PubMed: 31640756

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com