Recombinant Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP194] to MAGEA1 - C-terminal
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MAGEA1 antibody [SP194] - C-terminal
See all MAGEA1 primary antibodies -
Description
Rabbit monoclonal [SP194] to MAGEA1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human HeLa and A431 cell lysates. Human A431 cells. Human testis and skin squamous cell carcinoma tissues. Flow Cyt (Intra): SK-OV-3 cells.
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General notes
This product has switched from a hybridoma to recombinant production method on 23rd May 2023.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein A/G purified -
Purification notes
Purified from TCS by protein A/G. -
Clonality
Monoclonal -
Clone number
SP194 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab183309 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/400.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/400. Predicted molecular weight: 34 kDa.
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IHC-P |
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/400. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/400. Predicted molecular weight: 34 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. -
Tissue specificity
Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas. -
Sequence similarities
Contains 1 MAGE domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 4100 Human
- Omim: 300016 Human
- SwissProt: P43355 Human
- Unigene: 72879 Human
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Alternative names
- Antigen MZ2 E antibody
- Antigen MZ2-E antibody
- Cancer/testis antigen 1.1 antibody
see all
Images
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Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purified ab183309 at 1/20 dilution (8 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
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Immunohistochemical analysis of Human testis tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.
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Immunohistochemical analysis of Human skin squamous cell carcinoma tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.
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Flow cytometric analysis of Human A431 cells labeling MAGEA1 using ab183309(1/400) (green) compared to a negative control rabbit IgG (blue)
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Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309) at 1/400 dilution + Human A431 cell lysate
Predicted band size: 34 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab183309 has not yet been referenced specifically in any publications.