Recombinant
RabMAb

Recombinant Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

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Overview

  • Product name

    Anti-MAGEA1 antibody [SP194] - C-terminal
    See all MAGEA1 primary antibodies

  • Description

    Rabbit monoclonal [SP194] to MAGEA1 - C-terminal

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MAGEA1 aa 250 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P43355

  • Positive control

    • Human HeLa and A431 cell lysates. Human A431 cells. Human testis and skin squamous cell carcinoma tissues. FC: SK-OV-3 cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

     This product was previously labelled as MAGE 1

     

Properties

Applications

Our Abpromise guarantee covers the use of ab183309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/400. Predicted molecular weight: 34 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/400.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    May be involved in transcriptional regulation through interaction with SNW1 and recruiting histone deactelyase HDAC1. May inhibit notch intracellular domain (NICD) transactivation. May play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.

  • Tissue specificity

    Expressed in many tumors of several types, such as melanoma, head and neck squamous cell carcinoma, lung carcinoma and breast carcinoma, but not in normal tissues except for testes. Never expressed in kidney tumors, leukemias and lymphomas.

  • Sequence similarities

    Contains 1 MAGE domain.

  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen MZ2 E antibody
      • Antigen MZ2-E antibody
      • Cancer/testis antigen 1.1 antibody
      • cancer/testis antigen family 1, member 1 antibody
      • CT1.1 antibody
      • MAGA1_HUMAN antibody
      • MAGE 1 antigen antibody
      • MAGE-1 antigen antibody
      • MAGE1 antibody
      • MAGE1A antibody
      • MAGEA1 antibody
      • Melanoma antigen 1 antibody
      • Melanoma antigen family A 1 (directs expression of antigen MZ2 E) antibody
      • Melanoma antigen family A 1 antibody
      • Melanoma antigen MAGE 1 antibody
      • Melanoma associated antigen 1 antibody
      • Melanoma associated antigen MZ2 E antibody
      • Melanoma-associated antigen 1 antibody
      • MGC9326 antibody
      see all

    Images

    • Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

      Flow Cytometry analysis of SK-OV-3 (Human ovarian cancer epithelial cell) cells labeling MAGEA1 with purified ab183309 at 1/20 dilution (8 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

      Immunohistochemical analysis of Human testis tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

      Immunohistochemical analysis of Human skin squamous cell carcinoma tissue labeling MAGEA1 using ab183309 at a 1/100 dilution.

    • Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Flow Cytometry - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)

      Flow cytometric analysis of Human A431 cells labeling MAGEA1 using ab183309(1/400) (green) compared to a negative control rabbit IgG (blue)

    • Western blot - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Western blot - Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309)
      Anti-MAGEA1 antibody [SP194] - C-terminal (ab183309) at 1/400 dilution + Human A431 cell lysate

      Predicted band size: 34 kDa

    References

    ab183309 has not yet been referenced specifically in any publications.

    Publishing research using ab183309? Please let us know so that we can cite the reference in this datasheet.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com